IL-1β表达水平与广西肝癌家族聚集性的相关性

A research about relationship between IL-1β and familial aggregation of hepatocellular carcinoma in Guangxi area

目的:探究血清中细胞因子白细胞介素-1β(IL-1β)含量与广西肝癌流行区壮、瑶族肝癌家族聚集性的关系。方法:在广西肝癌流行地域收集肝癌高发120例作为实验组,选择近似相同生活背景、民族、HBsAg、性别、年龄相距±5岁的120例无癌家族成员为对照组,应用酶联免疫吸附(ELISA)技术检测两组共240例受检者外周血清 IL-1β含量。结果:不管从总体水平还是从民族、HBsAg携带情况、性别、年龄层面进行对比分析,实验组IL-1β含量均明显高于对照组,差异有统计学意义( P 均<0.05)。实验组中,先证者不同级别亲属之间IL-1β的含量差别无统计学意义(Z=1.648, P =0.642)。实验组中有 2~3 个肝癌先证者与有4个及以上肝癌先证者的家族成员外周血IL-1β含量相比较,差异无统计学意义(Z=-1.331, P =0.183)。结论:实验组入选者血清IL-1β因子检测含量明显高于对照组,促炎因子IL-1β高水平表达与广西的壮、瑶族肝癌家族聚集性存在关联性,IL-1β过量表达可能是肝癌高发家族成员罹患肝癌的易感因素之一。

Objective: To explore the relationship between serum cytokine interleukin(IL)-1β expression and familial aggregation of hepatocellular carcinoma(HCC) in Guangxi zhuang and yao population from high incidence regions of liver cancer. Methods: Enzyme-linked immunosorbent assay(ELISA) was used to examine serum IL-1β expression in 120 paired participants of cancer-free members from HCC families(the experimental group) and those (with the same living backgrounds,nationality,HBV infection,gender,age difference within 5 years as the matching conditions) from non-cancer families (the control group),at the endemic regions of liver cancer in Guangxi province,China. Results: In comparison with the overall expression or stratification (gender,nationality,age,HBsAg infection),the IL-1β expression in human sera were markedly higher in the HCC families members than that of the control members( P <0.05).In the experimental group,the serum levels of IL-1β among relatives of probands with different ranks in HCC families showed no statistical difference (Z=1.648, P =0.642).There was no significant difference in the serum levels of IL-1β in the peripheral blood among individuals with different number of liver cancer patients in the HCC families( Z=-1.331, P =0.183). Conclusion: There is a certain correlation between the elevated expression of IL-1β in carcinoma-free members from HCC high incidence families and the familial aggregation of HCC.