甘蓝型油菜光合基因的表达及其与含油量的关系

Expression of photosynthetic genes and their relationship with oil content in Brassica napus

以双低油菜高含油品系855和低含油品系868为材料,对9个光合基因(Bna0391450、Bna0449040、Bna0104450、Bna0501620、Bna0197350、Bna0305890、Bna0087590、Bna0084310、Bna0280620)在叶片、花蕾和种子中的表达及其与油分积累的相关性进行研究。结果表明:除Bna0280620基因在高油品系855中、Bna0084310基因在低油品系868授粉后15 d的种子中基本不表达外,其他基因在叶片、花蕾和授粉后不同时期的种子中均有表达,且基因表达量均呈先上升后下降的趋势;高油品系855中的基因表达量峰值出现在授粉后20 d;低油品系868中的基因表达量峰值出现在授粉后25 d;2个品系中,光合基因表达差异集中在苗期叶片和授粉后20 d的种子中,油分积累差异主要集中在授粉后30~40 d种子中。品系855中,除Bna0197350和Bna0280620外,油分积累与光合基因表达量均呈负相关,且相关性不显著;品系868中,除Bna0501620基因外,油分积累与光合基因表达量也呈负相关,相关性不显著。说明油菜光合基因在种子中有表达,但不影响种子中的油分积累量。

To study the correlation between photosynthetic genes expression and the oil accumulation, we selected 9 photosynthetic genes(Bna0391450, Bna0449040, Bna0104450, Bna0501620, Bna0197350, Bna0305890, Bna0087590,Bna0084310, Bna0280620) in leaves, flower buds and seeds of canola high oil line 855 and low oil line 868 as material..The results showed that except for the Bna0280620 gene in the high oil line 855 and the Bna0084310 gene in the seeds of the low oil line 868 after 15 days of pollination that was not expressed, the 9 genes were expressed in the leaves, flower buds and seeds at different stages after pollination. The expression of the genes increased first and then decreased. In the high oil line 855, the peak appeared 20 days after pollination, and the low oil line 868, the peak appeared 25 days after pollination. In the two lines, the difference in photosynthetic gene expression was concentrated in the seedling leaves and seeds at 20 days after pollination;the difference in oil accumulation was mainly 30-40 days after pollination. In addition to Bna0197350 and Bna0280620, there was a negative correlation between oil accumulation and photosynthetic gene expression in 855 with no significance. In addition to Bna0501620 gene, there was a negative correlation between oil accumulation and photosynthetic gene expression in 868 with no significance. It indicated that the photosynthetic gene of rapeseed was expressed in seeds, but did not affect the accumulation of oil in seeds.