基质金属蛋白酶13血清水平及基因启动子区-77A/G多态性与无症状性颈动脉狭窄的关联分析

Association of serum matrix metalloproteinase-13 levels and gene promoter -77A/G functional polymorphism with asymptomatic carotid stenosis

目的探讨基质金属蛋白酶13(MMP-13)血清水平及基因启动子区-77A/G多态性与无症状性颈动脉狭窄(asymptomatic carotid stenosis,ACS)的相关性。方法选取2015年6月-2017年12月期间在台州医院神经内科住院的621例ACS患者,通过颈动脉超声检查分为狭窄组(117例,狭窄≥50%)和对照组(504例,狭窄0~49%)。采用酶联免疫吸附试验方法检测所有患者MMP-13血清水平,运用聚合酶链反应-限制性片段长度多态性方法分析MMP13-77A/G多态性。结果狭窄组MMP-13血清水平与对照组相比差异有统计学意义(P<0.01)。狭窄组A等位基因携带者频率较对照组高,差异有统计学意义[OR=1.861;95%CI(1.398~2.478)]。经logistic回归分析校正多种危险因素后,狭窄组AG、AA基因型频率均较对照组高,差异有统计学意义[AG:OR=1.833,95%CI(1.108~3.033),AA:OR=3.197,95%CI(1.777~5.792)]。结论 MMP-13血清水平及其基因-77A/G多态性与中度以上狭窄的ACS的发病风险密切相关,-77A等位基因可能是发病的一个遗传易患因子。

Objective To explore the association of serum matrix metalloproteinase-13(MMP-13) levels and the gene promoter -77 A/G functional polymorphism with asymptomatic carotid stenosis(ACS). Methods A total of 621 ACS patients in Taizhou Hospital from June 2015 to December 2017 were divided into stenosis group(n=117,stenosis≥50%) and the control group(n=504, stenosis<50%) according to carotid ultrasound examination. Serum MMP-13 levels were measured by enzyme linked immunosorbent assay, and MMP13-77 A/G genotypes were determined by polymerase chain reaction-restriction fragment length polymorphim. Results The difference of serum MMP-13 levels between stenosis group and the control group were significant(P<0.01). The A allele frequency(MMP13-77 A/G polymorphism) was higher in the stenosis group [OR=1.861;95%CI(1.398-2.478)]. After adjustment for vascular risk factors binary logistic regression analysis showed that both AG and AA genotypes in stenosis group were significantly higher than those in the control group [AG:OR=1.833, 95% CI(1.108-3.033);AA:OR=3.197, 95%CI:1.777-5.792)]. Conclusion The serum level of MMP-13 and the gene-77 A/G polymorphism are closely related to the risk of ACS with carotid stenosis≥50%,-77 A allele may be a genetic predisposing factor for ACS.