摘要
研究了2种硫代葡萄糖苷标准品质谱裂解规律,发现硫代葡萄糖苷的β-D-硫代葡糖、磺酸肟及来源于氨基酸侧链R上的键都能够断裂发生重排现象从而形成碎片离子。在质谱裂解规律的基础上对3种不同品种的花椰菜叶中硫代葡萄糖苷进行结构鉴定。鉴定结果表明,3种花椰菜叶中含有8种硫代葡萄糖苷,包括3-(甲基亚磺酰基)丙基硫代葡萄糖苷(1)、4-(甲基亚磺酰)丁基硫代葡萄糖苷(2)、3-丁烯基硫代葡萄糖苷(3)、4-羟基苄基硫代葡萄糖苷(4)、1-羟基-吲哚-3-亚甲基硫代葡萄糖苷(5)、3-吲哚甲基硫代葡萄糖苷(6)、1-含氧甲基吲哚-3-亚甲基硫代葡萄糖苷(7)、6-(甲基磺酰)己基硫代葡萄糖苷(8)。其中,宝塔花椰菜叶中含有4种硫代葡萄糖苷(1,2,4,5),青花菜叶中含有6种硫代葡萄糖苷(2,3,5,6,7,8),紫色花椰菜叶中含有7种硫代葡萄糖苷(1,2,4,5,6,7)。
A method for identification of glucosinolate in three kinds of cruciferous vegetable leaves was developed by using H P LC- Q-TOF- MS.Firstly, the mass spectrometry fragmentation pathways of two standard glucosinolates was studied.The bond of glucose, sulfated thiohydroximate and R-group was easily fractured and the rearrangement reaction was observed for the forming the fragment ions.Then, the structure of 8 glucosinolates including 3-( methylsulfinyl) propyl ( 1 ), 4-( methyl-sulfinyl) butyl (2), 3-butenyl ( 3 ), 4-hydroxybenzyl ( 4 ), 1- hydroxyindol-3-ylmethyl (5).Indol-3-ylmethyl (6).1-methoxyindol- 3-ylmethyl (7), 6-( methylsulfonyl) hexyl glucosinolate (8) were identified based on the fragmentation pathways of two standard glucosinolates, There were 4 kinds of glucosinolate (1, 2 , 4, 5) in Baota Broccoli, 6 kinds of glucosinolate (2, 3 , 5 , 6 , 7 , 8) in Qinghuacai and 7 kinds of glucosinolate( 1, 2, 4, 5,6 .7) in Zise Broccoli.
作者
彭佩
田艳
邓放明
卿志星
PENG Pei;Tian yan;DENG Fang-ming;QING Zhi-jcing(College of Food Science and Technology , Hunan Agriculture University,Changsha , Hunan 410128, China)
出处
《食品与机械》
北大核心
2019年第4期42-47,119,共7页
Food and Machinery
基金
国家现代农业特色蔬菜产业技术体系专项(编号:CARS-24-E-02)
湖南省重点研发计划(编号:2016NK2110)
