HTLCS与HPLC测定滁菊中木犀草素、芹菜素、金合欢素方法的建立与比较

Establishment and comparison of HTLCS and HPLC for detecting the luteolin,apigenin and acacetin in Chuju chrysanthemum

以滁菊中木犀草素、芹菜素、合金欢素为测定指标,分别建立滁菊高效薄层扫描法(HTLCS)和高效液相色谱(HPLC)测定分析方法,并对每种方法线性关系和线性范围进行考察。结果表明:建立的HTLSC和HPLC法符合要求,线性关系良好,RE<3%,并且2种方法测定结果无显著性差异(P>0.05)。HTLCS法和HPLC法建立方法合理,能够采用HTLCS代替HPLC法进行滁菊中木犀草素、芹菜素、金合欢素3种成分的测定。

Taking the luteolin,apigenin and carnelin in the chrysanthemum as the determination index,the high-performance thin-layer scanning method(HTLCS)and high-performance liquid chromatography(HPLC)were used to determine the linear relationship between each method.The linear relationship and range of each method were investigated.The results showed that the established HTLSC and HPLC methods met the requirements,and the linear relationship was good,RE <3%.However,there was no significant difference between the two methods(P>0.05).The method of establishing HTLCS and HPLC method was reasonable.The determination of three components of luteolin,apigenin and acacetin could be carried out by using HTLCS instead of HPLC.