狗枣猕猴桃压缩饼干醇提物的抗辐射功能研究

Preparation and anti-radiation function of compressed biscuits with Actinidia kolomikta

超声波辅助60%的乙醇提取狗枣猕猴桃压缩饼干的活性成分;建立辐射诱导的氧化损伤模型,通过MTT法和流式细胞仪研究活性成分对肝细胞增殖能力以及细胞周期、细胞凋亡的影响,分析其抗辐射作用。结果显示,加药量达到70 mg/mL时对辐射诱导的肝细胞防护效果最佳,加药量达到60mg/mL时对辐射诱导的肝细胞修复效果最佳;通过细胞周期分析发现狗枣猕猴桃压缩饼干活性成分能够抑制辐射引起的细胞阻滞,使其恢复正常生长,防护作用较为显著;同时能够有效降低辐射引起的细胞凋亡率,对辐射引起的细胞损伤具有一定的防护作用。

The active components were extracted from compressed biscuits with Actinidia kolomikta .using 60% ethanol with ultrasonication.The oxidative damage of hepatocytes induced by ultraviolet ( UV) radiation was used as cell model.The effects of active components on proliferation.cell cycle, and apoptosis of hepatocytes were studied by MTT and flow cytometry.The results showed that the protective effect was the best when the dosage reached 70 mg/mL.while the therapeutic effect was the best when the dosage reached 60mg/mL.Through cell cycle analysis, it was found that the active components of compressed biscuits with Actinidia kolom ikt had an inhibiting effect on the cell cycle arrest caused by radiationand restoring its normal growth.and the preventive effects were obvious.Moreover, it could effectively reduce the apoptosis rate caused by radiation, and had a certain protective effect on cell damage.The compressed biscuits with Actinidia kolom ikt had anti-radiation function and provided a scientific basis for the further development and the utilization of Actinidia kolomikt.