高效液相色谱法测定毛茛中白头翁素含量的实验研究

Content determination of anemonin in Ranunculus japonicus Thunb. by high-performance liquid chromatography: An experimental study

目的:建立毛茛中白头翁素的含量测定方法。方法:采用Merck Hibar C_(18)色谱柱(4. 6mm×250mm,5μm),流动相为甲醇-水(30∶70),检测波长220nm,流速0. 8ml/min,柱温30℃,进样量20μL。理论板数按白头翁素计≥4000。结果:白头翁素在0. 342～8. 108mg/ml范围内线性关系良好,平均回收率为98. 7%(n=9),相对标准偏差(RSD)为1. 2%。结论:该方法简单、精密度高,结果准确可靠,可作为毛茛中白头翁素含量测定的一种有效方法。

Objective:To establish a method for content determination of anemonin in Ranunculus japonicus Thunb.Methods:High-performance liquid chromatography was performed on a Merck Hibar C(s column(4.6mm×250mm,5μm)with a mobile phase of methanol-water(30:70)at a detection wavelength of 220nm,a flow rate of 0.8ml/min,a column temperature of 30℃,and a sample size of 20(μL.The number of theoretical plates for anemonin was≥4000.Results:Anemonin showed a good linear relationship within the range of 0.342~8.108mg/ml,with an average recovery rate of 98.7%(n=9)(relative standard deviation=1.2%).Conclusion:This method is simple and accurate with reliable results and can be used as an effective method for the con tent determination of anemonin in Ranunculus jap on icus Thunb.