miR-29b和miR-126在糖尿病新生血管性青光眼虹膜组织中的表达及其意义

Expression and Significance of miR-29b and miR-126 in Iris Tissues of Diabetic Neovascular Glaucoma

【目的】探讨微小核糖核酸-29b(miR-29b)、微小核糖核酸-126(miR-126)在糖尿病(DM)新生血管性青光眼(NVG)虹膜组织中的表达及其意义。【方法】选取100例本院确诊为DM合并NVG患者100例(DM-NVG组),选取同期在本院诊治的单纯青光眼患者54例(NVG组),另外选取健康虹膜组织捐献者15例为对照组(CON组)。检测并比较三组miR-29b和miR-126表达水平,并分析其与DM合并NVG患者临床病理特征的关系,采用logistic多元回归分析DM合并NVG的危险因素。【结果】DM-NVG组和NVG组虹膜组织中miR-29b表达水平均显著低于CON组(P<0.05),miR-126表达水平显著高于CON组(P<0.05);DM-NVG组虹膜组织中miR-29b表达水平显著低于NVG组(P<0.05),miR-126表达水平显著高于NVG组(P<0.05)。虹膜组织中miR-29b表达与眼压、视力指数、血管内皮生长因子(VEGF)均相关(P<0.05);miR-126表达与眼压、VEGF相关(P<0.05)。DM合并NVG患者虹膜组织中miR-29b与miR-126表达水平呈显著负相关(P<0.05);多元回归分析显示:VEGF、DM类型、miR-29b和miR-126是导致DM并发NVG疾病的危险因素(P<0.05)。【结论】miR-29b在DM患者 NVG虹膜组织中低表达,miR-126高表达,其可能参与DM患者NVG的发生发展,为其临床上靶向治疗提供参考。

【Objective】 To observe the expression of micro RNA-29b (miR-29b) and micro RNA-126 (miR-126) in the iris tissues of diabetes mellitus (DM) neovascular glaucoma (NVG) in order to explore their significance in the pathogenesis and development of DM with NVG.【Methods】One hundred cases of DM complicated with NVG were selected as DM-NVG group, 54 cases of typically simple glaucoma were selected as glaucoma group (NVG group) and 15 cases of healthy iris tissue donors were as control group (CON group). The expression levels of miR-29b and miR-126 were detected by fluorescence quantitative polymerase chain reaction (PCR). The relationship between the two genes' expression with the clinicopathological characteristics of patients with DM combined with NVG was analyzed. Pearson correlation analysis was used to analyze the relationship between miR-29b and miR-126 expression in the iris tissue of patients. Logistic multiple regression analysis was used to analyze the risk factors of NVG in DM patients.【Results】The expression of miR-29b in the iris tissues of the DM-NVG group and NVG group was significantly lower than that of the control group (P<0.05). However, the expression of miR-126 in the DM-NVG and NVG groups was significantly higher than that of the CON group (P<0.05). The expression of miR-29b in the iris of DM-NVG group was significantly lower than that of the NVG group (P<0.05), and its expression of miR-126 was significantly higher than that of the NVG group (P<0.05). The expression of miR-29b in iris tissues was related to intraocular pressure, visual acuity index and VEGF (P<0.05). The expression of miR-126 was related to intraocular pressure and VEGF (P<0.05). There was a significant negative correlation between miR-29b and miR-126 expression in iris tissues of patients with DM combined with NVG (P<0.001). Multivariate regression analysis showed that VEGF, DM type, miR-29b and miR-126 were the risk factors for DM complicated with NVG (P<0.05).【Conclusion】The expression of miR-29b is low in the iris tissues of diabetic neovascular glaucoma, while miR-126 is highly expressed, which may be involved in regulating the occurrence and development of diabetic neovascular glaucoma. Thus,it provides a reference for clinical treatment of diabetic neovascular glaucoma.