结直肠癌组织中HOXB7分子的表达及其临床意义

Expression of HOXB7 molecule in colorectal cancer tissues and its clinical significance

目的探讨HOXB7mRNA及其蛋白在结直肠癌(CRC)组织中的表达及其临床意义。方法采用RT-PCR法检测6例结直肠癌旁组织(ACRM组织)和6例CRC组织中HOXB7mRNA的表达,采用免疫组织化学染色法检测30例ACRM组织和98例CRC组织中HOXB7蛋白的表达,并分析CRC组织中HOXB7蛋白的表达与患者临床病理特征和生存预后的关系。结果RT-PCR法检测结果表明,CRC组织中HOXB7mRNA的表达水平高于ACRM组织(P=0.003)。免疫组织化学染色法检测结果表明,CRC组织中HOXB7蛋白的表达阳性率高于ACRM组织(P<0.05)。HOXB7蛋白的阳性表达与肿瘤浸润深度、淋巴结转移及TNM分期密切相关(P<0.05)。生存曲线分析结果显示,HOXB7蛋白表达阳性组的生存情况与表达阴性组的差异无统计学意义(P=0.865)。结论HOXB7蛋白的阳性表达可作为评估CRC进展的新型生物学指标,但HOXB7蛋白对于CRC预后的评估价值有待于进一步考证。

Objective To investigate the expression and clinical significance of HOXB7 mRNA and protein in colorectal cancer (CRC) tissues. Methods The expressions of HOXB7 mRNA were evaluated in 6 cases of adjacent colorectal mucosal (ACRM) tissues and 6 cases of CRC tissues by using RT-PCR. The HOXB7 protein expressions were evaluated in 30 cases of ACRM tissues and 98 cases of CRC tissues by using immunohistochemistry. The correlations between the expression of HOXB7 protein, and the clinicopathologic factors or the patient’ survival were analyzed. Results RT-PCR results showed that expression level of HOXB7 mRNA in CRC tissues was significantly higher than that of ACRM tissues (P=0.003). Immunohistochemistry results showed that significantly higher positive-expression rate of HOXB7 protein in CRC tissues compared with ACRM tissues (P<0.05). Positive expression of HOXB7 protein was associated with depth of tumor invasion, lymph node metastasis, and the TNM stage (P<0.05). Kaplan-Meier survival curves showed that positive expression of HOXB7 protein was not inversely correlated with survival of CRC patients (P=0.865). Conclusion Positive expression of HOXB7 protein is a novel biomarker for estimating the progression of CRC, but remains of textual research may be to confirm the significance of HOXB7 protein for prognosis evaluation.