黄芩素对胃癌细胞增殖、凋亡的影响及与c-Jun氨基末端激酶信号通路的关系

Effects of Baicalin on proliferation and apoptosis of gastric cancer cells and its relationship with c-jun amino-terminal kinase signaling pathway

目的基于c-Jun氨基末端激酶信号通路观察黄芩素对胃癌AGS细胞增殖及凋亡的影响。方法以膜联蛋白V(Annexin V)染色检测黄芩素干预后胃癌AGS细胞凋亡,进而用细胞计数试剂盒(CCK-8)检测黄芩素干预对胃癌AGS细胞增殖的影响。通过蛋白质印迹法(Western blot)检测黄芩素干预后c-Jun氨基末端激酶信号通路下游信号分子B淋巴细胞瘤-2、c-Jun蛋白表达。应用SPSS18.0统计软件分析,计量资料以均值±标准差(Mean±SD)表示,组间差异采用单因素方法或t检验分析,计数资料采用χ^2检验。结果CCK-8检测结果显示,经0μmol/L(对照组)、25、50、100μmol/L的黄芩素干预48h后,对应4组胃癌AGS细胞增殖活力分别为100.00±3.38、79.49±4.47、68.67±2.26、57.02±3.28。与对照组比较,黄芩素干预能够显著降低胃癌AGS细胞的增殖活力,且呈剂量依赖性(P<0.05)。Annexin V染色和流式细胞仪验证,经0μmol/L(对照组)、25、50、100μmol/L的黄芩素干预48h后,对应4组胃癌AGS细胞凋亡比例为1.97±0.72、3.44±0.45、4.11±0.35、4.93±1.03。与对照组比较,黄芩素可显著促进胃癌AGS细胞凋亡(P<0.05)。Westernblot法显示黄芩素干预后胃癌AGS细胞B淋巴细胞瘤-2和c-Jun蛋白的表达均呈不同程度的降低。结论黄芩素干预显著抑制胃癌细胞活力、诱导其凋亡而抑制胃癌细胞生长,具有下调B淋巴细胞瘤-2和c-Jun表达的作用。

Objective Effects of Baicalein on AGS cell proliferation and apoptosis in gastric cancer based on c-Jun N-terminal kinase signaling pathway. Methods Firstly, Annexin V staining was used to detect the apoptosis of AGS cells after baicalin intervention, and then cell counting kit-8 (CCK-8) was used to detect the effect of baicalin intervention on the proliferation of AGS cells after gastric cancer. Finally, Western blotting was used to detect the expression of c-jun amino-terminal kinase signaling molecules B lymphoma-2 and c-jun protein in the downstream of c-jun amino-terminal kinase signaling pathway after the intervention of baicalin. Results CCK-8 assay showed that after 48 h of baicalin intervention with 0 μmol/L (control group), 25, 50, 100 μmol/L, the proliferation activity (Mean±SD) of AGS cells in the corresponding four groups was 100.00±3.38, 79.49±4.47, 68.67±2.26, 57.02±3.28. respectively. Compared with the control group, baicalin intervention can significantly reduce the proliferation activity of AGS cells in gastric cancer with a dose-dependent effect, P<0.05. After 48 hours of intervention with baicalin at 0 μmol/L (control group), 25, 50, 100 μmol/L, Annexin V staining and flow cytometry, the apoptosis rates (Mean±SD) of AGS cells in the four groups were 1.97±0.72, 3.44±0.45, 4.11±0.35, 4.93±1.03. Compared with the control group, baicalin can significantly promote the apoptosis of AGS cells, P<0.05. Western blotting analysis showed that the expressions of B lymphocytoma-2 and c-Jun in AGS cells were decreased after baicalin treatment. Conclusion Baicalein significantly inhibited the activity of gastric cancer cells, induced apoptosis of gastric cancer cells and inhibited the growth of gastric cancer cells. And it’s significant The role of modulating bcl-2 and c-jun expression.