三磷酸腺苷合酶α亚基及人表皮生长因子受体在大肠癌患者中的表达及意义

Significance and expression of adenosine triphosphate synthase subunit alpha and human epidermal growth factor receptor in colorectal cancer

目的探讨三磷酸腺苷合酶α亚基(ATP5A1)、人表皮生长因子受体-2(Her-2)及Her-3在结大肠癌中的表达及意义。方法收集2008年5月至2018年8月烟台市中医院外科手术切除的100例经病理确诊为大肠癌的石蜡标本,采用免疫组织化学法检测100例大肠癌及癌旁正常组织中ATP5A1、Her-2及Her-3的表达,并分析三者与年龄、性别、肿瘤大小、肿瘤部位、Dukes分期、分化程度,淋巴结转移的关系。应用SPSS 17.0统计软件分析,计量资料采用Fisher精确概率法及χ2检验,相关性分析采用Spearman相关分析。结果ATP5A1在大肠癌组织中(46/100)的阳性表达显著高于癌旁正常组织(0/100),差异有统计学意义(P<0.01),Her-2在大肠癌组织中(56/100)的阳性表达显著高于癌旁正常组织(14/100),差异有统计学意义(P<0.01),Her-3在大肠癌组织中(50/100)的阳性表达显著低于癌旁正常组织(6/100),差异有统计学意义(P<0.01)。大肠癌组织中ATP5A1、Her-2及Her-3阳性表达率与Dukes分期、淋巴结转移密切相关(P<0.05),与年龄、性别、肿瘤大小、肿瘤部位无明显相关(P>0.05)。经Spearman相关性分析,大肠癌组织中ATP5A1与Her-2表达呈正相关(r=0.659,P<0.01),ATP5A1与Her-3表达呈正相关(r=0.546,P<0.01),Her-2与Her-3表达呈正相关(r=0.878,P<0.01)。结论高表达的ATP5A1、Her-2及Her-3促进大肠癌的发生、浸润转移,三者联合检测有助于大肠癌的诊断。

Objective To explore significance and expression of adenosine triphosphate synthase subunit alpha (ATP5A1), human epidermal growth factor receptor-2 (Her-2) and Her-3 in colorectal cancer. Methods The expression of ATP5A1, Her-2 and Her-3 in 100 cases of colorectal cancer and adjacent normal tissues was detected by immunohistochemical method. The relationship between ATP5A1, Her-2 and Her-3 and age, sex, tumor size and tumor location, Dukes stage, differentiation degree, lymph node metastasis was analyzed. Results The positive rate of ATP5A1 in colorectal cancer (46/100) was higher than that in adjacent normal tissues (0/100)(P<0.05). The positive rate of Her-2 in colorectal cancer (56/100) was higher than that in adjacent normal tissues (14/100)(P<0.05). The positive rate of Her-3 in colorectal cancer (56/100) was higher than that in adjacent normal tissues (6/100)(P<0.05). The positive rate of ATP5A1, Her-2 and Her-3 in poorly differentiated was higher than that in the high and moderately differentiated (P<0.05). The positive rate of ATP5A1, Her-2 and Her-3 in C+ D stage was higher than that in A+ B stage (P<0.05). The positive rate of ATP5A1, Her-2 and Her-3 in lymph node metastasis was higher than that in without lymph node metastasis (P<0.05). The positive expression rates of ATP5A1, Her-2 and Her-3 in colorectal cancer were independent of age, sex, tumor size and tumor location. The expression of ATP5A1 was positively correlated with the expression of Her-2 in colorectal cancer (r=0.659, P<0.05). The expression of ATP5A1 was negatively correlated with the expression of Her-3 in colorectal cancer (r=0.546, P<0.05). The expression of Her-2 was negatively correlated with the expression of Her-3 in colorectal cancer (r=0.878, P<0.05). Conclusion High expression of ATP5A1, Her-2 and Her-3 played an important role in the occurrence, invasion and metastasis of colorectal cancer. The combined detection of these three protein could play a guiding role in the diagnosis of colorectal cancer.