7/8肾切除构建Ⅳ型心肾综合征模型

Experimental establishment of cardio-renal syndrome type Ⅳ model

目的:探讨7/8肾切除制备稳定的Ⅳ型心肾综合征模型的可行性。方法:(1)采用7/8肾切除手术制备Sprague Dawley大鼠慢性肾脏病模型,测定血压、血清肌酐、血尿素氮及肌酐清除率,Masson染色观察肾组织纤维化情况。(2)通过心脏超声观察肾切除术后1、3、5周的心脏形态变化。(3)心肌组织进行麦胚芽凝集素染色、Masson染色及心肌肥厚指标表达测定。结果:7/8肾切除后第5周,大鼠出现血压明显升高、血清肌酐及尿素氮升高,肌酐清除率降低,同时血清磷及成纤维细胞因子23升高;肾组织形态学上表现为间质纤维化。心脏表现为左心室心肌壁增厚、质量指数增加;心肌肥厚指标心房钠尿肽及脑尿钠肽上调;心肌细胞横截面积增大、心肌组织呈现结构紊乱和间质纤维化。结论:Sprague Dawley大鼠7/8肾切除术后第5周成功制备稳定的Ⅳ型心肾综合征模型。

Objective:To investigate the viability of establishing stable model of cardio-renal syndrome type Ⅳ. Methodology:(1) 7/8 nephrectomy were performed on Sprague Dawley rats for chronic kidney disease model,blood pressure,serum creatinine,serum urea nitrogen and creatinine clearance rate were monitred,Masson staining was used to observe the fibrosis of renal tissue.(2) Cardiac morphology at 1,3,and 5 weeks after nephrectomy was observed by echocardiogram.(3) The cardiac tissue was selected for wheat germ agglutinin staining,Masson staining and the expression measurement of cardiac hypertrophy indicators. Results:5 weeks after 7/8 nephrectomy,rats in surgery group showed significant rise in blood pressure,serum creatinine and blood urea nitrogen,accompanied by decreased creatinine clearance rate and interstitial fibrosis in remnant kidney,serum phosphorus and fibroblast growth factor 23 were increased as well.Left ventricular myocardial wall thickening and mass index increased significantly,the expression of cardiac hypertrophy indicators(atrial natriuretic peptide and brain natriuretic peptide),myocardial interstitial fibrosis and myocyte cross section area were markedly increased. Conclusion:5 weeks after 7/8 nephrectomy,the stable model of cardio-renal syndrome type Ⅳ can be successfully established in Sprague Dawley rats.