两种栀子挥发油的GC-MS成分分析

Determination of Essential Oil from the Two Gardeniae Fructus by GC-MS

采用水蒸气蒸馏法提取两个产地栀子中的挥发油,并通过气-质联用的方法测定其挥发性化学成分,色谱柱为DB-5MS弹性石英毛细管柱(30m×0.25mm,0.25μm),载气为氦气,流速1.0mL/min,进样口温度280℃,三阶程序升温,进样方式为分流进样,分流比20∶1,进样量1μL.从云南和河南样品中分别鉴定了27个和20个化合物的结构.用峰面积归一化法进行相对定量,质量分数超过5%的成分有N-甲基吡咯烷酮、乙酸丁酯、邻苯二甲酸二丁酯、己二酸二(2-乙基己基)酯等.

The essential oil from two Gardenia jasminoides Ellis was extracted by water distillation extraction method. The chemical constituents were separated and identified with GC-MS. Quartz capillary column DB-5MS(30 m×0.25 mm,0.25 μm). The carrier gas was helium and the flow rate was 1.0 mL/min. The temperature of injection port was 280℃.Temperature rises with three order programmed route.The injection method was split-flow and the ratio of split-flow was 20∶ 1.1 μL sample was injected. The compounds were identified from Yunnan and Henan samples were 27 and 20 respectively. The relative contents of components were determined by area normalization method. The mass fractions exceed five percent include:2-pyrrolidinone 1-methyl;acetic acid butyl ester;dibutyl phthalate and hexanedioic acid, bis(2-ethylhexyl) ester.