酵母硒对杜寒杂交羊生长性能、血常规和血清生化指标的影响

Effects of Selenium Yeast on Growth Performance,Blood Routine and Serum Biochemical Indexes of Dorper×Thin-Tailed Han Crossbred Sheep

本试验旨在探讨饲粮中添加酵母硒对杜寒杂交羊生长性能、血常规和血清生化指标的影响。选用40只体重(34kg左右)相近、健康状况良好的杜寒杂交断奶羔羊,随机分为4组,每组10只,进行单栏单只饲养。各组试验羊分别饲喂在基础饲粮中添加0(对照)、0.2、0.4和0.8mg/kg的硒(硒的形式为酵母硒)的试验饲粮,对应的试验饲粮中硒含量的实测值分别为0.074(对照)、0.307、0.491和0.846mg/kg。预试期10d,正试期20d。结果显示:随着硒添加量的增加,平均日增重呈先增加后降低的趋势,在硒添加量为0.2mg/kg时平均日增重最大。当硒添加量为0.2mg/kg时,杜寒杂交羊的料重比最低,极显著低于其他3个组(P<0.01)。当硒添加量为0.8mg/kg时,血小板计数最高,极显著高于其他各组(P<0.01)。当硒添加量为0.8mg/kg时,嗜碱性粒细胞百分数最高,极显著高于0.4mg/kg组(P<0.01)。饲粮中添加不同水平的硒对其他血常规指标均无显著影响(P>0.05)。当硒添加量为0.8mg/kg时,血清中总蛋白、尿素氮和肌酐含量均最高,显著或极显著高于其他各组(P<0.05或P<0.01)。0.8mg/kg组血清中谷丙转氨酶和谷草转氨酶的活性最高,显著或极显著高于对照组(P<0.05或P<0.01)。对照组血清中碱性磷酸酶的活性最高,与0.2mg/kg组差异不显著(P>0.05)。当硒添加量为0.8mg/kg时,血清中γ-谷氨酰转移酶的活性最高,极显著高于其他各组(P<0.01)。当硒添加量为0.8mg/kg时,血清中磷含量最高,极显著高于0.2和0.4mg/kg组(P<0.01)。当硒添加量为0.8mg/kg时,血清中铁含量最低,极显著低于对照组(P<0.01),同时0.4mg/kg组血清中铁含量也极显著低于对照组(P<0.01)。综合本试验测定指标,杜寒杂交羊饲粮中酵母硒形式硒的适宜添加量为0.2mg/kg(饲粮中硒含量的实测值为0.307mg/kg)。

This experiment was conducted to study the effects of dietary selenium yeast on the growth performance,blood routine and serum biochemical indexes of Dorper×thin-tailed Han crossbred sheep.Forty Dorper×thin-tailed Han crossbred weaning lambs with the similar body weight (about 34 kg) and good health were randomly assigned to 4 groups with 10 sheep in each group,and all sheep were housed in individual pen.Sheep in the 4 groups were fed experimental diets supplemented with 0 (control),0.2,0.4 and 0.8 mg/kg selenium (the form was selenium yeast) based on a basal diet,and the measured values of the selenium content in the experimental diets were 0.074 (control),0.307,0.491 and 0.846 mg/kg,respectively.The experiment lasted for 10 days for adaption,and 20 days for test.The results showed as follows: average daily gain (ADG) was firstly increased and then decreased with selenium supplemental level increasing,and the highest value of ADG was found when selenium supplemental level was 0.2 mg/kg.The lowest value of feed/gain (F/G) was found when selenium supplemental level was 0.2 mg/kg,and it was significantly lower than that in the other three groups ( P <0.01).The highest platelet count was found when selenium supplemental level was 0.8 mg/kg ,and it had significant difference compared with the other three groups ( P <0.01).The highest basophilic granulocytes (BASO) percentage was found when selenium supplemental level was 0.8 mg/kg,and it had significant difference compared with the 0.4 mg/kg group ( P <0.01).Dietary supplemented with different levels of selenium had no significant effects on other blood routine indexes ( P >0.05).The highest values of serum total protein (TP),urea nitrogen (UN) and creatinine (CREA) contents were found when selenium supplemental level was 0.8 mg/kg,and they had significant differences compared with the other groups ( P <0.05 or P <0.01 ).The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in 0.8 mg/kg group were the highest,which were significantly higher than those in the control group ( P <0.05 or P <0.01).The serum alkaline phosphatase activity in control group had the highest value,and it had no significant difference compared with 0.2 mg/kg group ( P >0.05).The highest value of serum γ-glutamyltransferase (γ-GGT) activity was found when selenium supplemental level was 0.8 mg/kg,and it had significant difference compared with the other groups ( P <0.01).Phosphonium content in serum was the highest when selenium supplemental level was 0.8 mg/kg,and it had significant difference compared with 0.2 and 0.4 mg/kg groups ( P <0.01 ).Iron content in serum was the lowest when selenium supplemental level was 0.8 mg/kg,which was significantly lower than that in control group ( P <0.01),and it in 0.4 mg/kg group was also significantly lower than that in control group ( P <0.01).Considering all indexes of this experiment,the appropriate selenium (the form is selenium yeast) supplemental level is 0.2 mg/kg in the diet of Dorper×thin-tailed Han crossbred sheep,and the measured value of selenium content in the diet is 0.307 mg/kg.