视黄醇对仔猪睾丸支持细胞体外生物学特性的影响

Effects of retinol on in vitro biological characteristics of sertoli cells from prepubertal porcine testis

支持细胞是构成曲细精管上皮的重要组成细胞,视黄醇及其衍生物是雄性动物睾丸发育和精子发生中所必须的物质,支持细胞是睾丸内视黄醇及其衍生物作用的靶细胞。本研究以3~5周龄仔猪睾丸支持细胞为材料,通过分析视黄醇对体外支持细胞活力、增殖和凋亡相关基因表达、相关细胞因子转录及合成的影响,评价视黄醇对支持细胞体外生物学特性的影响。结果显示,与对照组相比,视黄醇添加浓度达到5.000、0.125μmol·L-1时,显著抑制培养24h和72h的细胞活性(P<0.05);添加1.250μmol·L-1视黄醇组和对照组相比,增殖相关基因PCNA、BCL-2和C-MYC显著下调(P<0.05),凋亡相关基因BAX显著上调(P<0.05),且支持细胞内GDNF、CSF-1和EGF在基因表达和蛋白质水平上均显著降低(P<0.05)。体外培养条件下,添加视黄醇抑制支持细胞活性,促进细胞凋亡。

Sertoli cells are principal components of testicular seminiferous tubule epithelium.Retinol and its derivatives also play a critical role in the regulation of testicular development and spermatogenesis through sertoli cell mediation.To explore the effect of retinol on the in vitro biological characteristics of testicular sertoli cells,sertoli cells from 3 to 5 week-old porcine testis were selected as materials.Cellular viability,the expression of proliferation-related and apoptosis-related genes and secretion of cytokines were assessed.The results showed that compared with the control group,when the concentration of retinol was 5.000 and 0.125 μmol ·L-1 ,significant effects were seen on sertoli cell viability after 24 h and 72 h ( P <0.05).Adding 1.250 μmol ·L-1 retinol group compared with the control group,the proliferation-related genes PCNA,BCL-2 and C-MYC were significantly down-regulated ( P <0.05),apoptosis-related genes BAX was significantly up-regulated ( P <0.05),and support intracellular GDNF,CSF-1 and EGF were significantly reduced in gene expression and protein levels ( P <0.05).In summary,the addition of retinol can inhibit the activity of supporting cells and promote apoptosis in vitro culture conditions.