甲基硒酸对口腔癌细胞增殖、迁移、凋亡和周期的作用研究

Influence of MSA on Proliferation,Migration,Apoptosis,and Cell Cycle of Oral Squamous Cell Carcinoma Tca8113 and KB Cells

目的:利用甲基硒酸(methylseleninic acid,MSA)作用于口腔鳞状细胞癌Tca8113和KB细胞,探讨MSA对口腔癌细胞的增殖、迁移、凋亡和周期的影响。方法:体外培养口腔癌细胞,加入不同浓度的MSA培养不同时间,MTS 法及细胞克隆法测定细胞增殖情况;流式细胞仪检测细胞凋亡率和细胞周期分布;划痕实验测定细胞的迁移情况。结果: MSA可显著抑制口腔癌细胞的增殖和迁移,作用效果随药物浓度增加而增强;MSA可诱导口腔癌细胞体外凋亡,随着药物浓度的增加,口腔癌细胞凋亡率逐渐增加。MSA可改变口腔癌细胞的细胞周期,将口腔癌细胞周期阻断在G 2期,并且减少G 1期和S期细胞的比例。结论: MSA可显著抑制口腔癌细胞的体外增殖、迁移,并促进其凋亡,改变口腔癌细胞周期。MSA有望成为治疗口腔癌的一种新型药物。

Objective: To explore the influence of MSA on the proliferation,migration,apoptosis,and cell cycle of oral squamous cell carcinoma Tca8113 and KB cells. Methods: MTS assay was used to detect cell proliferation activity.Wound healing assay was used to detect the capability of cell migration.The cell cycle distribution and apoptotic rate were measured using flow cytometry assay. Results: MSA inhibited the proliferation of Tca8113 and KB cells and reduced the cell migration.MSA suppressed the apoptosis of Tca8113 and KB cells markedly in a dose dependent manner.Cell cycle was blocked at G2 stage and the number of cell at G1 and S stage was reduced when stimulated with MSA. Conclusions: MSA can inhibit the proliferation and migration,induce the apoptosis,and change cell cycle of oral squamous cell carcinoma Tca8113 and KB cells in vitro .MSA is expected to be a new method to prevent and treat the oral cancer.