IL-1β、MMP-2在大鼠牙根吸收组织中的表达及意义

Expression of Interleukin-1β and Matrix Metalloproteinase-2 in Periodontal Tissues of Rat during Root Resorption

目的:分析白介素-1β(IL-1β)、基质金属蛋白酶-2(MMP-2)与大鼠牙根吸收组织吸收程度的相关性。方法: 30只Wistar 大鼠随机分为 3组,每组10只。A组为空白对照组,不做处理,B组于右侧上颌切牙和第一磨牙间置持续性压低重力作用装置施以 40 g力,C组于相同位置施以100 g力。组织学观察牙根吸收程度,并于加力前、加力 1、7、14、21、28 d后,采用酶联免疫法检测三组龈沟液IL-1β、MMP-2。结果: HE染色显示A组未见明显牙根吸收,牙周膜纤维致密,排列规则;B组直接骨吸收,牙槽骨边缘可见成骨细胞呈立方状排列,成骨活跃;C组牙周纤维排列较紊乱,根分叉处牙根吸收,牙根吸收达牙本质层。施力前,3组IL-1β、MMP-2水平比较差异无统计学意义( P >0.05);施力1 d后,A组IL-1β、MMP-2水平分别为(108.7±7.25) mg/L、(658.3±28.9)μg/L,B组分别为(165.2±12.4) mg/L、(702.3±30.4)μg/L,C组分别为(225.7±16.4) mg/L、(845.7±46.4)μg/L,差异均有统计学意义( P <0.05),其中C组显著高于B组、A组,且于第7天达到高峰。结论: IL-1β、MMP-2水平参与牙根吸收,对其进行监测有利于对牙根吸收诊断及防治工作提供新思路。

Objective: To analyze the expression of interleukin-1β and matrix metalloproteinase-2 in the periodontal tissues of rat during root resorption. Methods: Thirty Wistar rats were randomly divided into three groups,each with ten rats.Group A was the blank control group,and no treatment was done.Group B was applied 40g force on the right upper incisor and the first molar,and group C was applied 100g force at the same position.The degree of root absorption of the teeth was observed by histologically.Before and after 1,7,14,21,and 28 days,interleukin-1β and matrix metalloproteinase-2 of gingival crevicular fluid were detected by enzyme-linked immunoassay. Results: HE staining showed no obvious root absorption in group A,with dense periodontal membrane fibers and regular arrangement and bone resorption in group B,and the osteoblasts were cubic arranged at the margin of alveolar bone.In group C,periodontal fibers were disordered,and the root was absorbed at the bifurcation point and absorbed to the dentin layer.Before applying the force,there was no statistically significant difference in expression of interleukin-1β and matrix metalloproteinase-2 among three groups ( P >0.05).After 1 day of force application,interleukin-1β and matrix metalloproteinase-2 levels in group A were (108.7±7.25)mg/L and(658.3±28.9)μg/L,(165.2±12.4)mg/L,and (702.3±30.4)μg/L in group B,(225.7±16.4)mg/L and(845.7±46.4)μg/L in group C.The differences were statistically significant ( P <0.05),and group C was significantly higher than group B and group A,reaching peak on the 7th day. Conclusion: The levels of nterleukin-1β and matrix metalloproteinase-2 are involved in root resorption,and monitoring them can provide new ideas for root resorption diagnosis and prevention.