大鼠脊髓损伤后脊髓组织中上调差异基因的生物信息学分析

Bioinformatics Analysis of Up-regulated Differential Genes in Rats post Spinal Cord Injury

目的探究大鼠脊髓损伤后脊髓组织中差异表达的上调基因及功能。方法采用改良Allen法制备雌性Sprague-Dawley大鼠脊髓损伤模型,应用基因芯片技术检测在大鼠脊髓损伤后脊髓中差异基因的变达,运用基因本体论和基因组百科全书数据库分析差异上调基因、功能定位与通路。结果脊髓组织总核糖核酸质量检测结果合格;基因芯片检测结果显示上调差异基因1874个,下调差异基因2348个;生物信息学分析上调差异基因的生物学过程、细胞组分和分子功能,涉及凋亡、免疫反应、炎症等,通路分析主要涉及磷脂酰肌醇三激酶-蛋白激酶B信号通路和Toll样受体信号通路等。结论脊髓损伤后,上调差异表达基因可能参与脊髓损伤后的继发性反应,如炎症、免疫应答、缺氧等,继而影响脊髓损伤后的运动功能和感觉功能。

Objective To investigate the differential expression and gene functions of up-regulated genes in rats with spinal cord injury. Methods Female Sprague-Dawley rats' model of spinal cord injury was established with the modified Allen's method. Gene chip technology was used to detect the variation of differentially expressed genes in the spinal cord after spinal cord injury in rats.The differences in genes,functional localization and pathways were analyzed with gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway database. Results The results of total RNA quality in spinal cord segment were qualified.Gene chip results showed that there were 1874 differentially up-regulated genes and 2348 differentially down-regulated genes.Bioinformatics was used to analyze differentially up-regulated genes in terms of biological processes,cellular components,and molecular functions.The differentially up-regulated genes were involving apoptosis,immune response,inflammation,etc., pathway analysis mainly showed the differentially up-regulated genes involved phosphoinositide 3-kinase protein kinase B signaling pathway and Toll-like receptor signaling pathways. Conclusion Differentially up-regulated genes may be involved in secondary reactions following spinal cord injury,such as inflammation,immune response and hypoxia,and then further affect motor function and sensory function.