HBV cccDNA研究现状与挑战

Research status and challenges of HBV cccDNA

现有的抗病毒治疗方案很难清除HBV感染而达到"完全"治愈,导致许多患者需要长期、甚至终身接受核苷(酸)类似物抗病毒治疗,其根本的原因是肝细胞核内存在具有转录活性的共价闭合环状DNA(cccDNA)。为了让读者了解HBV cccDNA方面的研究进展,本期邀请了国内权威专家,就抗HBV治疗与慢性乙型肝炎的功能性治愈、靶向HBV cccDNA的药物及生物技术、HBV cccDNA转录调控机制与抗HBV治疗前景、HBV cccDNA的体外细胞模型和实验小鼠模型、原位杂交技术在检测HBV核酸和cccDNA中的应用、HBV cccDNA定量检测方法等6个方面的研究进展分别加以介绍。

Current antiviral treatment regimens seldom clear hepatitis B virus(HBV) infection and achieve complete cure,and therefore,many patients need long-term or even life-long antiviral therapy with nucleos(t) ide analogues.The root cause is the presence of covalently closed circular DNA(cccDNA) with transcriptional activity within hepatocytes.In order to help readers understand the research advances in HBV cccDNA,this issue invites leading experts in China to introduce the research advances from the following six aspects: anti-HBV treatment and functional cure of chronic hepatitis B,drugs and biotechniques targeting HBV cccDNA,transcriptional regulation of HBV cccDNA and prospects of anti-HBV treatment,in vitro cell models and experimental mouse models of HBV cccDNA,application of in situ hybridization in detection of HBV nucleic acid and cccDNA,and quantification of HBV cccDNA.