原位杂交技术在检测HBV核酸和cccDNA中的应用

Application of in situ hybridization in the detection of hepatitis B virus nucleic acids and cccDNA

原位杂交技术(ISH)是分子生物学、组织化学及细胞学相结合而产生的一门新兴技术,能够在细胞和染色体水平上对特定核酸进行定量与定位,广泛应用于病毒学研究。ISH对于HBV中的核酸(RNA、复制中间体DNA),以及共价闭合环状DNA的检测有着重要意义,就这一技术的发展以及在HBV研究中的应用作梳理与总结。

In situ hybridization(ISH) is a new technique which combines molecular biology,histochemistry,and cytology.It can quantify and locate specific nucleic acids at the cellular and chromosomal levels and is widely used in virological research.ISH is of great significance for the detection of hepatitis B virus(HBV) nucleic acids(RNA and replicative intermediate DNA) and covalently closed circular DNA.This article reviews the development of ISH and its application in HBV research.