辐射交联制备含白芷香豆素的水凝胶及其性能研究

Preparation of hydrogel containing Angelica dahurica coumarin by radiation crosslinking and its properties

以白芷香豆素辐照抑菌稳定性研究为基础,采用辐射交联与冻/融循环方法制备含白芷香豆素的聚乙烯醇/羧甲基纤维素钠水凝胶材料。通过抑菌率、力学强度、吸水率及扫描电镜观察等方法研究香豆素含量及薄荷脑、小分子胶原肽复配对水凝胶结构与性能的影响。结果表明,白芷香豆素具有辐照稳定性,经辐照至剂量0、10和20kGy后,其对大肠杆菌的最小抑菌质量浓度(MIC)分别为3.13、3.13和1.56mg/mL,抑菌性提高;对金黄色葡萄球菌的MIC均为6.25mg/mL;复合水凝胶中,随香豆素含量增大,水凝胶抑菌率先快速增大后逐渐减缓,拉伸强度逐渐降低;当添加质量分数为1%时,通过与薄荷脑复配可将水凝胶抑菌率提高到100%,而拉伸强度无明显变化,为0.7MPa;小分子胶原肽可提高白芷香豆素的分散性和抑菌率,拉伸强度略有降低,水凝胶的2、24h吸水率分别达到800%和1100%。

A polyvinyl alcohol/sodium carboxymethylcellulose hydrogel material containing Angelica dahurica coumarin was prepared by radiation crosslinking and freeze / thraw cycles. The effects of coumarin content and conbination of menthol and low-molecular-weight collagen on the structure and properties of the hydrogel were studied through bacteriostatic tests as well as tensile strength, water absorption, and scanning electron microscope observation. The results showed irradiation stability for angelica dahurica coumarin. The minimum inhibitory concentrations (MICs) of angelica dahurica coumarin to E. coli were 3.13, 3.13, and 1.56 mg/mL at absorbed doses of 0, 10, and 20 kGy, respectively, thereby proving that the antibacterial property increased with dosage. The MIC of angelica dahurica coumarin to S. aureus was 6.25 mg / mL. The bacteriostasis rate of the composite hydrogels increased rapidly at first and then slowed down with the increasing coumarin contents although their tensile strength decreased. The bacteriostasis rate of the hydrogel reached 100% on introducing menthol at a mass fraction of 1%;the tensile strength reached a value of 0.7 MPa showing no obvious change. Low-molecular-weight collagen improved the dispersibility and bacteriostasis rate of coumarin. The tensile strength of the hydrogels decreased slightly;the water absorption of the hydrogels reached 800% and 1 100% in 2 and 24 h, respectively.