面向荧光剂药代动力学的动态扩散荧光层析系统

Dynamic Diffuse Fluorescence Tomography System for Fluorescence Pharmacokinetics

为捕捉生物组织体内快速变化的荧光信号,并借鉴扩散荧光层析在穿透深度、高灵敏度等方面的优势,设计了一套具备快速实时采样能力的动态扩散荧光层析成像在体测量系统.该系统采用多光源-探测器布配方案,结合光电倍增管探测器与锁相光子计数检测技术,具有高空间采样密度、高探测灵敏度、大动态测量范围以及高性价比等优势.有效性评估实验表明:该系统具备良好的稳定性和很强的环境光抑制能力,频率串扰率低于1.39%.仿体成像实验表明:该系统能够有效地对单个和多个目标体成像.活体健康小鼠吲哚菁绿肝代谢实验进一步表明:该系统可有效捕捉小鼠体内快速变化的荧光信号,能够用于荧光剂药代动力学研究.

To capture the fast-changing fluorescence information during a finite metabolism process in vivo,a dynamic system with the ability of parallel detection was developed based on the merits of high penetration depth and sensitivity of diffuse fluorescence tomography.It adopts the mode of muti-source and muti-detector,and combines photo-multiplier tube detector and lock-in photon-counting technique to achieve high spatial sampling density,high sensitivity,wide dynamic range,as well as better cost-effectiveness.The assessment results show that this system has good performances in stability and ambient light suppression,and the crosstalk-rate of frequency belows 1.39%.The phantom experiment demonstrates the favorable abilities of imaging single and multiple targets.Furtherly,the in vivo experiment on indocyanine green metabolism in healthy mouse liver demonstrates the capability of capturing the fast-changing fluorescence signals in small animals for fluorescence pharmacokinetic study.