黔产青钱柳化学成分及α-葡萄糖苷酶抑制活性研究

Study on chemical constituents and α-glucosidase inhibitory activity of Cyclocarya paliurus in Guizhou province

为了阐明青钱柳中的降血糖功效成分,实验对黔产青钱柳进行了系统化学成分研究和α-葡萄糖苷酶抑制活性评价。利用各种色谱学手段和波谱学方法,从青钱柳叶80%乙醇提取物中分离鉴定出13个化合物,分别为阿福豆苷(1)、山柰酚3-O-(4″-O-乙酰基)-α-L-吡喃鼠李糖苷(2)、juglanoside J(3)、1α,2α,4β-3羟基-1,2,3,4-四氢萘酮(4)、青钱柳苷III(5)、青钱柳苷J(6)、黄体酮(7)、去酰基萝藦苷元(8)、20-乙酰氧基-4-烯-3-酮(9)、齐墩果酸(10)、5-O-P香豆酰奎宁酸甲酯(11)、亚麻酸甲酯(12)、软脂酸(13)。其中,化合物9为新天然产物,化合物2~4、7、8、11、13均为首次从该植物中分离得到。用PNPG法对化合物1~10进行了α-葡萄糖苷酶抑制活性筛选,结果显示,化合物1、2有较强的α-葡萄糖苷酶抑制活性,表明黄酮类成分是青钱柳降血糖功效的物质基础之一。

In order to elucidate the hypoglycemic components of C.paliurus,the chemical constituents and the inhibitory activity of α-glucosidase were systematic studied.13 compounds were isolated and identified from 80% ethanol extract of C.paliurus leaves by means of various chromatographic methods and spectroscopic methods.These compounds were characterized as afzelin(1),Kaempferol 3-O-(4″-O-acetyl)-α-L-rhamnopyranoside(2),juglanoside J(3),1α,2α,4β-triol-1,2,3,4-tetrahydronaphthalene(4),Cyclocarioside III(5),Cyclocarioside J(6),Progesterone(7),12-O-deacetylmetaplexigenin(8),20α-acetoxypregn-4-en-3-one(9),Oleanolic acid(10),5-O-P-coumaroylquinic acid methyl ester(11),Methyl linolenate(12),Palmitic acid(13).Among them,compound 9 was a new natural product;compounds 2-4,7,8,11,and 13 were isolated from this plant for the first time.The inhibitory activity of α-glucosidase was screened by PNPG method;the results showed that compounds 1 and 2 have strong α-glucosidase inhibitory activity.These results indicated that flavonoid is one kind of hypoglycemic active components of C.paliurus plant.