番石榴叶化学成分分离鉴定及psiguadial D的抗癌活性研究

Study on the chemical constituents from Psidium guajava leaves and antitumor activity of psiguadial D

本文对番石榴Psidium guajava叶的化学成分进行分离鉴定并对部分化合物抗肿瘤活性进行评价。采用正相硅胶、Sephadex LH-20、MCI柱色谱和制备液相色谱等方法对番石榴叶甲醇提取物的化学成分进行分离纯化,运用现代波谱技术鉴定了13个化合物,分别为4,5-diepipsidial A(1)、psidial A(2)、guajadial(3)、psiguadial A(4)、psiguadial D(5)、α-生育酚(6)、亚油酸(7)、槲皮素-3-O-β-D-吡喃木糖苷(8)、槲皮素-3-O-α-L-吡喃阿拉伯糖苷(9)、槲皮素-3-O-α-L-呋喃阿拉伯糖苷(10)、β-谷甾醇(11)、儿茶素(12)、没食子酸(13)。其中化合物1为首次从天然产物中分离得到。化合物5对人体肿瘤细胞株HL-60、SMMC-7721、A-549、MCF-7、SW-480均显示出较为明显的抑制活性,半数抑制浓度为4.08~11.23μmol/L。

The aim of this research was to investigate the chemical constituents from Psidium guajava Leaves and anticancer activity of selected compound.Thirteen compounds were obtained from the methanolic extracts of leaves of Psidium guajava by Prep.HPLC and column chromatography on silica gel,Sephadex LH-20,and MCI.On the basis of spectroscopic analysis,the isolated compounds were identified as 4,5-diepipsidial A(1),psidial A(2),guajadial(3),psiguadial A(4),psiguadial D(5),α-tocopherol(6),linoleic acid(7),quercetin-3-O-β-D-xylopyranoside(8),quercetin-3-O-α-L-arabinopyranoside(9),quercetin-3-O-α-L-arabinofuranoside(10),β-sitosterol(11),catechin(12),and gallic acid(13).Compound 1 was isolated from a natural source for the first time.Compound 5 was evaluated for their cytotoxicity in vitro against human tumor HL-60,SMMC-7721,A-549,MCF-7,and SW-480 cell lines and exhibited inhibitory effects on all five cells,with IC50 values in the range of 4.08-11.23 μmol/L.