竹黄菌与竹红菌的化学成分及细胞毒活性比较研究

Chemical constituents of Shiraia bambusicola and Hypocrella bambusae and their cytotoxic activity

为研究竹黄菌与竹红菌化学成分及细胞毒活性的差异,本研究通过高效液相色谱(HPLC)分析结合常规色谱方法,分离鉴定了两种真菌的6个相同成分,分别为3个主要成分竹红菌甲素(1)、竹红菌乙素(2)和竹红菌丙素(3),以及3,6,8-三羟基-1-甲基口山酮(7)、3,8-二羟基-6-甲氧基-1-甲基口山酮(8)和过氧麦角甾醇(9)。另外,从竹黄菌中还分离得到11,11′-二去氧沃替西林(5)、麦角甾-7,22E-二烯-3β,5α,6β-三醇(10)和麦角甾-7,22E-二烯-2β,3α,9α-三醇(11),并首次从竹红菌中分离得到竹红菌丁素(4)、灰黄霉素(6)、化合物7和8。活性筛选发现,化合物5对三株肿瘤细胞NCI-H1975、HepG2和MCF-7有很强细胞毒活性,化合物1有较强细胞毒活性,而化合物6活性较弱。

To investigate the differences of chemical composition and cytotoxic activity between two medicinal fungi,Shiraia bambusicola and Hypocrella bambusae,high performance liquid chromatography analysis and chromatographic separations were conducted.Six compounds were isolated from both fungi and characterized as hypocrellin A(1),hypocrellin B(2),hypocrellin C(3),3,6,8-trihydroxy-1-methylanthone(7),3,8-dihydroxy-6-methoxy-1-methylanthone(8) and ergosterol peroxide(9),respectively.In addition,11,11′-dideoxyverticillin(5),ergosta-7,22E-dien-3β,5α,6β-triol(10) and ergosta-7,22E-diene-2β,3α,9α-triol(11) were also obtained from S. bambusicola,while hypocrellin D(4),(+)-griseofulvin(6),compounds 7 and 8 were reported from H. bambusae for the first time.Compound 5 showed potent inhibitory activity against human lung adenocarcinoma cell(NCI-H1975),human carcinoma(HepG2) and human breast cancer cells(MCF-7).Compound 1 also showed significant inhibitory activity against the three cell lines.In contrast,the cytotoxic activity of compound 6 was relatively weak.