羊角天麻化学成分及β-羟高铁血红素形成抑制活性研究

Chemical constituents of Dobinea delavayi and their β-hematin formation inhibition activity

为探究羊角天麻(Dobinea delavayi)的β-羟高铁血红素形成抑制活性成分,本实验采用硅胶、Sephadex LH-20柱色谱等方法进行化合物的纯化分离,通过理化性质及波谱数据鉴定化合物结构。结果从羊角天麻中分离得到12个化合物,分别鉴定为3,4,2′,4′-四羟基二氢查尔酮(1)、紫铆花素(2)、3,4,2′,4′,α-五羟基查尔酮(3)、金色草素(4)、芒果苷(5)、没食子酸(6)、二十四亚甲基环阿尔廷醇(7)、6β-羟基-豆甾-4-烯-3-酮(8)、β-谷甾醇(9)、胡萝卜苷(10)、棕榈酸甲酯(11)、1-棕榈酸单甘油酯(12),其中化合物1~4、6~8均为首次九子母属植物中分离得到。活性研究结果显示,化合物1~6均具有β-羟高铁血红素形成抑制活性,其中黄酮类化合物2~4具有较强的抑制活性,其IC50分别为120.3、61.5、56.0μg/mL。

To study the inhibitory active constituents from Dobinea delavayi against β-hematin formation.The compounds were isolated by silica gel and sephadex LH-20 colunm chromatography,and their structures were identified by the analyses of their physicochemical properties and NMR data.Twelve compounds were isolated and identified as 3,4,2′,4′-tetrahydroxydihydrochalcone(1),butin(2),3,4,2′,4′,α-pentahydroxychalcone(3),aureusidin(4),mangiferin(5),gallic acid(6),24-metbylenecycloartanol(7),6β-hydroxystigmast-4-en-3-one(8),β-sitosterol(9),daucosterol(10),methyl hexadecanoate(11),and glyceroylmonopalmitate(12).Compounds 1-4 and 6-8 were isolated from the Dobinea for the first time.According to the bioassay experiment,compounds 1-6 showed β-hematin formation inhibition activities in different degrees,and the flavonoids compounds 2-4 revealed significant β-hematin formation inhibition activities with IC50 of 120.3,61.5 and 56.0 μg/mL,respectively.