Tiam1持续过表达促进多脏器细胞增殖能力的体内研究

In vivo study on Tiam1 continuous overexpression for promoting cell proliferation of multiple organs

目的以T淋巴瘤侵袭转移诱导因子1(Tiam1)转基因小鼠及裸鼠为实验载体,探讨Tiam1过表达在细胞增殖中的功能及作用。方法将Tiam1^+/+转基因小鼠及野生型ICR小鼠多器官组织取材、包埋、制片并进行病理形态学观察,免疫组织化学检测增殖标志物Ki-67的表达;利用裸鼠皮下成瘤实验观察Tiam1过表达对结直肠癌细胞株HT29的体内增殖能力的影响。结果Tiam1+/+转基因小鼠的结直肠、胃、肺、肾脏以及睾丸组织均较野生型小鼠表现出更为旺盛的增殖能力;Tiam1稳定过表达能够显著促进结直肠癌细胞株HT29裸鼠皮下肿瘤增殖能力,差异具有统计学意义(P<0.01)。结论Tiam1持续过表达能够在体内水平促进小鼠多脏器细胞及结直肠癌细胞的增殖能力。

Objective To investigate the playing function and effect of Tiam1 in cell proliferation with Tiam1 transgenic mice and nude mice as the experimental vectors. Methods The multiple organs tissues of Tiam1^+/+ transgenic mice and wild ICR mice conducted sampling,embedding,preparing section and pathologic morphologic observation.The cell proliferation ability was detected by combining proliferation marker Ki-67 immunohistochemistry.The influence of Tiam1 overexpression on the in vivo proliferation ability of colorectal cancer cell line HT29 was observed by using the nude mice subcutaneous tumor formation experiment. Results The tissues of colorectum,stomach,lung,kidney and testis of Tiam1 transgenic mice showed more vigorous proliferation ability compared with the wild mice.The Tiam1 stable overexpression of colorectal cancer cell line HT29 could significantly promote the ability of nude mice subcutaneous tumor proliferation,and the difference was statistically significant( P <0.01). Conclusion Continuous overexpression of Tiam1 can promote in vivo proliferation ability of mice multiple organs cells and colorectal cancer cells,which provides the new target point and direct and powerful theoretical basis for the deep mechanism of cellular proliferation and tumor progression.