广西莪术水提物对卵形鲳鲹细胞免疫力的影响

Effects of Curcuma kwangsiensis S.G.Lee et C.F.Liang on Immune Activities of Trachinotus ovatus Cells

卵形鲳鲹(Trachinotus ovatus)是我国重要的海水养殖鱼类,经济价值极高,但近年来各种鱼病频繁爆发造成了巨大损失。为探究广西莪术(Curcuma kwangsiensis S.G.Leeet C.F.Liang)水提物对卵形鲳鲹免疫力的影响,本文在细胞水平上,利用卵形鲳鲹肾脏组织细胞系研究广西莪术水提物的细胞毒性,以确定其细胞安全浓度。然后利用卵形鲳鲹细胞系研究广西莪术水提物对总一氧化氮合酶(T-NOS)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)和溶菌酶等卵形鲳鲹细胞免疫相关酶的影响。结果表明,广西莪术水提物的细胞安全浓度为≤5mg/mL,广西莪术水提物对卵形鲳鲹细胞的免疫相关酶活力(总一氧化氮合酶、酸性磷酸酶、超氧化物歧化酶、溶菌酶)有明显促进作用。广西莪术有潜力发展成为一种高效的水产免疫增强剂,并且基于传统壮药积极开展绿色无药物残留的新型抗水产病害中药制剂的研究,对于促进水产养殖业持续健康发展,确保水产品质量和食品安全意义重大。

Trachinotus ovatus is an important marine fish with high economic value in China.However,the frequent outbreak of various fish diseases has caused great losses in recent years.In order to investigate the effects of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immunity of T.ovatus,at the cellular level,the cytotoxicity of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang in Guangxi was studied using the ovate-shaped kidney tissue cell line to determine the safe concentration of cells.Then,the effect of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immune-related enzymes of ovate-shaped cells which included total nitrogen monoxide synthase (T-NOS),acid phosphatase (ACP),superoxide dismutase (SOD) and lysozyme was studied by using the ovate cell line.The safe concentration of Curcuma kwangsiensis S.G.Lee et C.F.Liang water extracts was ≤5 mg/mL.Curcuma kwangsiensis S.G.Lee et C.F.Liang could obviously promote the activity of immune-related enzymes (T-NOS,ACP,SOD and lysozyme) in TOK cells.Curcuma kwangsiensis S.G.Lee et C.F.Liang has potential to develop into a highly effective aquaculture immune enhancer.Based on the traditional Chinese Zhuang medicine,it is of great significance to carry out the research on new Chinese herbal preparations against aquatic diseases,which could promote the sustainable and healthy development of aquaculture and ensure the quality of aquatic products and food safety.