高血压合并左心室肥厚患者血清miRNA-29a-3p水平与心肌纤维化的相关性

Relationship between miRNA-29a-3p and myocardial fibrosis in patients of hypertension with left ventricular hypertrophy

目的探讨高血压伴左心室肥厚患者miRNA-29a-3p的水平与心肌纤维化程度的相关性。方法纳入高血压2级以上患者160例,分为单纯高血压组80例和高血压合并左心室肥厚组80例,同时入选健康对照组90例。检测3组血清miRNA-29a-3p、Ⅰ型胶原、Ⅲ型胶原水平和基质金属蛋白酶9水平,分析高血压合并左心室肥厚组患者血清miRNA-29a-3p与Ⅰ型胶原、Ⅲ型胶原和基质金属蛋白酶9的相关性。结果单纯高血压组和高血压合并左心室肥厚组血清miRNA-29a-3p水平高于对照组,高血压合并左心室肥厚组miRNA-29a-3p水平又高于单纯高血压组(P<0.05);单纯高血压组和高血压合并左心室肥厚组血清Ⅰ型胶原、Ⅲ型胶原和基质金属蛋白酶9水平均高于对照组,高血压合并左心室肥厚组血清Ⅰ型胶原、Ⅲ型胶原和基质金属蛋白酶9水平均又高于单纯高血压组(P<0.05)。高血压合并左心室肥厚组患者血清miRNA-29a-3p表达水平与心肌纤维化3项指标Ⅰ型胶原、Ⅲ型胶原和基质金属蛋白酶9水平均呈正相关(P<0.05)。结论miRNA-29a-3p水平可反映高血压合并左心室肥厚患者的心肌纤维化程度。

Objective To explore the relationship between miRNA-29a-3p and myocardial fibrosis in patients of hypertension with left ventricular hypertrophy. Methods One hundred and sixty patients with grade 2 or higher primary hypertension were divided into pure hypertension group(80 cases) and hypertension with left ventricular hypertrophy group(80 cases),the healthy people in our hospital physical examination center were enrolled as control group(90 cases).The miRNA-29a-3p,matrix metalloproteinases-9(MMP-9),procollagen Ⅰ and Ⅲ in serum were detected in three groups.The relationship between miRNA-29a-3p and MMP-9,PINP and PIIINP in hypertension with left ventricular hypertrophy group were analyzed. Results Serum miRNA-29a-3p levels in the hypertension group and hypertension with left ventricular hypertrophy were higher than those in the control group,and the levels of miRNA-29a-3p in the hypertension with left ventricular hypertrophy group were higher than those in the hypertension group( P < 0.05).The levels of serum type Ⅰ collagen,type Ⅲ collagen and matrix metalloproteinase-9 in the hypertension group and hypertension with left ventricular hypertrophy were higher than those in the control group.The levels of serum type Ⅰ collagen,type Ⅲ collagen and matrix MMP-9 in hypertension with left ventricular hypertrophy group were higher than the hypertension group( P < 0.05).The expression level of serum miRNA-29a-3p in hypertension with left ventricular hypertrophy was positively correlated with the levels of type Ⅰ collagen,type Ⅲ collagen and matrix MMP-9( P < 0.05). Conclusion Level of miRNA-29a-3p reflects the degree of myocardial fibrosis in patients with hypertension with left ventricular hypertrophy.