子宫内膜异位症患者子宫内膜组织中Rab5、Smad2基因表达及其临床意义

Expression and clinical significance of Rab5 and Smad2 in endometrium of patients with endometriosis

目的探讨子宫内膜异位症患者子宫内膜组织中Rab5、Smad2基因表达及其临床意义。方法选取行腹腔镜手术治疗的患者364例,其中术后诊断为子宫内膜异位症的182例为观察组,子宫肌瘤的182例为对照组。比较2组血清CA125、炎性因子、血清子宫内膜抗体的表达情况,并对观察组中r-AFS分期者的上述指标进行比较。Westernblot检测组织中Rab5和Smad2的表达情况。观察血清中CA125、炎性因子、血清子宫内膜抗体与组织中Rab5和Smad2的表达的相关性。采用Logistic回归分析确定子宫内膜异位症的危险因素。结果观察组血清CA125、炎性因子、血清子宫内膜抗体水平均明显高于对照组(P<0.05)。观察组组织中Rab5和Smad2的蛋白表达明显高于对照组(P<0.05)。Rab5和Smad2的表达与CA125、炎性因子、血清子宫内膜抗体均呈正相关(P<0.05)。Logistic回归分析显示,子宫内膜组织中Rab5和Smad2表达是子宫内膜异位症患者的危险因素。结论子宫内膜异位症患者子宫内膜组织中Rab5和Smad2表达明显升高,与相应的临床指标呈正相关。

Objective To study the expression of Rab5 and Smad2 in endometrial tissues of patients with endometriosis and analyze their clinical significance. Methods A total of 364 patients treated with laparoscopic surgery were selected as the research objects.Among them,182 patients diagnosed with endometriosis after laparoscopic surgery were taken as the observation group,and 182 patients with uterine fibroids admitted to our hospital during the same period were taken as the control group.The expressions of serum CA125,inflammatory factors and serum endometrial antibodies were compared between these two groups.The above indexes of different r-AFS staging groups were compared.Western blot was used to detect the expression of Rab5 and Smad2 in tissue.The patients′ clinical indicators were observed and their correlation with Rab5 and Smad2 was analyzed to make the receiver operating characteristic curve.Logistic multiple regression analysis of related factors of endometriosis. Results The levels of CA125,inflammatory factors and serum endometrial antibodies in the observation group were significantly higher than those in the control group in serum.The expression of Rab5 and Smad2 in the observation group was significantly higher than that in the control group( P < 0.05).The expression of Rab5 and Smad2 was positively correlated with CA125,inflammatory factors and serum endometrial antibodies( P < 0.05).Logistic multiple regression analysis showed that Rab5 and Smad2 in endometrial tissues were risk factors for patients with endometriosis. Conclusion The expression of Rab5 and Smad2 in endometrial tissues of patients with endometriosis is significantly increased,which is positively correlated with the corresponding clinical indicators and is a risk factor for endometriosis.