通过遗传多样性小鼠筛选EV71致病相关基因

Screening of EV71 pathogenicity-related genes using genetic diversity mice

目的 通过遗传多样性小鼠资源(Genetic Diversity Mice Resources)筛选肠道病毒71型(Enterovirus 71,EV71)的致病相关基因,深入理解该病毒的致病机理,对临床诊断、治疗以及预后提供基础。方法 10 7copies EV71病毒经腹腔感染4~6周龄遗传多样性小鼠品系,收集血液和后肢骨骼肌样本,利用实时荧光定量PCR技术分析病毒载量,之后利用The GeneMine系统遗传学数据库分析感染数据,筛选获得EV71致病相关基因。结果 通过遗传多样性小鼠感染数据筛选到53个与EV71致病相关的基因。结论 遗传多样性小鼠在模拟人群基因多样性、研究复杂性状疾病等方面具有较大的优势,是筛选致病相关基因理想的动物新资源。

Objective The aim of this study was to screen enterovirus 71(EV71)pathogenicity-related genes using genetic diversity mice to further understand the pathogenesis of the EV71,and lay the foundation for clinical diagnosis,treatment and prognosis.Methods Four-to-six week old genetic diversity mice were challenged with 10 7 copies of enterovirus 71 by intraperitoneal injection.The blood and hind limb skeletal muscles were collected,and the viral load was determined by real-time quantitative PCR.Then,the GeneMine system genetic database was used to analyze the infection data,and EV71 pathogenicity-related genes were screened.Results Fifty-three genes related to EV71 infection were screened using genetic diversity mouse infection data.Conclusions Genetic diversity mice have significant advantages for simulating population genetic diversity and are a new resource to study complex trait diseases.Most importantly,they are an ideal and efficient resource for the screening of pathogenicity-related genes.