尾静脉注射M1细胞构建BALB/c裸鼠髓系白血病模型

Establishment of a nude mouse model of granulocytic leukemia by injection of M1 cells into the tail vein

目的 应用BALB/c裸小鼠构建M1白血病模型。方法 将12只5~6周龄的雌性BALB/c Nude小鼠随机分为低、中、高剂量组及空白组,每组3只,进行尾静脉注射。低、中、高三组分别接种对数生长期M1细胞悬液每只1×10^6个、每只5×10^6个及每只8×10^6个。观察小鼠的一般状况,于造模后第0、10、20、30天采集外周血,检测血常规、进行白细胞分类,第30天或濒死时处死取材,流式检测外周血及骨髓CD33CD117阳性细胞比例,制备组织病理切片。结果 模型组小鼠在接种后第10~15天出现萎靡少动、脊背弓起等状况。实验第30天时,与空白组相比,高剂量组小鼠外周血白细胞数显著升高(P<0.05);各组小鼠外周血白血病细胞比例较空白组显著增高(P<0.05);各组小鼠骨髓中CD33^+CD117^+比例增高,高剂量组最为显著(P<0.05);高剂量组脾脏中可见少量白血病细胞浸润。结论 BALB/c Nude小鼠经尾静脉注射8×10^6个小鼠白血病细胞M1后可构建急性髓系白血病模型,符合急性髓系白血病的生物学特点。

Objective To construct a nude mouse model of granulocytic leukemia with M1 cells.Methods Nine female BALB/c nude mice,5-6 weeks old,were randomly divided into three groups(n=3 per group):group A mice were the blank control group,and groups B and C were inoculated via the tail vein with a logarithmic growth phase M1 cell suspension(1×10^6 and 5×10^6,respectively).The general condition of the mice was observed.Blood samples,peripheral blood leukocyte differential count,and peripheral blood CD33CD117 positive cells were detected on days 0,10,20 and 30 after modeling.On the day 30 or at the time of death,tissue sections were taken for pathological examination.Results The mice in the model group developed symptoms such as wilting and dorsiflexion on days 10-15 after inoculation.Compared with the blank group,the number of peripheral blood leukocytes in each mouse injected with 8×10 6 M1 cells was significantly increased on the 30th day(P<0.05).On the 30th day,the proportion of peripheral blood leukemia cells in the model group was significantly higher than that in the blank group(P<0.05),Compared with the blank group,the proportion of CD33^+CD117^+cells in the bone marrow of the other groups increased,and the high dose group was the most significant.(P<0.05),and that on day 30,leukemia cells infiltrated into the spleen of model mice.Conclusions The injection of 8×10^6 mouse leukemia M1 cells into the tail vein of BALB/c nude mice can induce an acute myeloid leukemia model consistent with the biological characteristics of acute myeloid leukemia.