鸡粪添加木质素对平邑甜茶根区土壤硝酸盐代谢的影响

Effects of adding lignin to chicken manure on nitrate metabolism in soil of the root zone of Malus hupehensis Rehd

为研究鸡粪和木质素处理对平邑甜茶根区土壤硝酸盐代谢的影响,采用盆栽实验,添加质量分数12.5%鸡粪、12.5%的鸡粪+木质素1.5g/kg、12.5%的鸡粪+木质素2.5g/kg的处理,在春、夏、秋三个季节分别取样,分析土壤硝酸盐代谢变化规律。结果表明,施用鸡粪和木质素比单施鸡粪抑制了土壤铵态氮向硝态氮转化的速率,降低了土壤硝化强度,施加木质素越多抑制效果越明显,在各个季节对反硝化强度影响不同。木质素提高了土壤硝酸还原酶和亚硝酸还原酶活性以及土壤铵态氮的含量,降低了土壤硝态氮的含量。施用木质素能明显抑制土壤氮素流失,该研究为木质素的合理应用提供了理论依据。

In order to study the effects of chicken manure and lignin treatment on nitrate metabolism in soil of the root zone of Malus hupehensis Rehd., the changes of nitrate metabolism in soil were analyzed by using pot experiments, with the treatment of adding mass fraction 12.5% chicken manure, 12.5% chicken manure+1.5g/kg lignin and 12.5% chicken manure + 2.5 g/kg lignin, sampling in the spring, summer, and autumn. Results showed that the application of chicken manure and lignin inhibited the transformation rate of ammonium nitrogen to nitrate nitrogen in soil compared with single application of chicken manure, reduced the nitrification intensity of soil. The more lignin was applied, the more obvious the inhibitory effect was. And the effect on the denitrification intensity of soil was different in each season. Lignin increased the activity of nitrate reductase and nitrite reductase in soil, as well as increased the content of ammonium nitrogen in soil, and reduced the content of nitrate-nitrogen in soil. The application of lignin could significantly inhibit the loss of nitrogen in soil, which provided a theoretical basis for the rational application of lignin.