柴胡皂苷b2对大鼠体内柴胡皂苷a肝组织分布的影响

Effect of Saikosaponin b2 on Liver Distribution of Saikosaponin a in Rats

研究柴胡皂苷b2对柴胡皂苷a在大鼠肝脏组织中分布的影响。将Wistar大鼠随机分为两组,分别灌胃给药柴胡皂苷a(25mg/kg)和柴胡皂苷a+柴胡皂苷b2(25+3.125mg/kg)混合药液,然后于0.25、0.5、1、2、4h收集肝脏,肝组织匀浆经液液萃取处理后通过LC-MS/MS法测定肝组织中柴胡皂苷a的含量。给药后0.5h,柴胡皂苷a在肝组织中分布量最大,并随时间延长其含量迅速下降。柴胡皂苷b2显著增加0.5h时柴胡皂苷a在肝组织的累积量,而对其他时间点柴胡皂苷a在肝组织的累积量无显著性影响。本研究表明柴胡皂苷b2增加了柴胡皂苷a在大鼠肝脏的分布,可能对药效产生影响。

The effect of saikosaponin b2 on liver distribution of saikosaponin a in rats was investigated.Wistar rats were randomly divided into two groups.one group was orally administered saikosaponin a(25mg/kg)independently and the other group was treated by combinative administration of saikosaponin a and saikosaponin b2(25+3.125 mg/kg).The livers were collected at 0.25,0.5,1,2 and 4 h,respectively.The tissue samples were extracted with liquid-liquid extraction and the content of saikosaponin a in liver tissue was determined by LC-MS/MS method.The distribution of saikosaponin a in liver reached the maximum at 0.5h after administration and then decreased rapidly with time.Furthermore,saikosaponin b2 significantly increased the accumulation of saikosaponin a in liver tissue at 0.5h,but it had no significant difference at other time points.This study indicated that saikosaponin b 2 could enhance the liver distribution of saikosaponin a,which may have an effect on the drug efficacy.