HPLC测定青叶胆中獐芽菜苦苷含量

Determination of Swertiamarins Content of Swertia mileensis Using HPLC

獐牙菜苦苷是青叶胆中主要的抗肝损伤成分,采用HPLC法测定青叶胆中獐芽菜苦苷含量,色谱柱:Zorbax SB-C18(4.6mm×150mm,5μm);流动相:甲醇:水(25:75);检测波长:236nm;流速为1.0mL/min;柱温:25℃。獐牙菜苦苷在2.4~21.6μg的范围内线性关系良好,加样回收率良好。本实验方法快速、简便、稳定,可用于青叶胆有效成分獐牙菜苦苷的含量测定。

Swertiamarin is the main anti-liver injury component in Swertia mileensis.The content of swertiamarins in Swertia mileensis was determined by HPLC,using chromatographic column of Zorbax SB-C 18 column(4.6mm×150mm,5μm)with mobile phase of methanol-water(25:75),detection wavelength of 236 nm,flow rate of 1.0 mL/min,and column temperature of 25℃.The swertiamarins showed a good linear relationship within the range of 2.4~21.6μg.The method is fast,simple and stable,and can be used to determine the content of swertia in Swertia mileensis.