Six4蛋白在结肠癌中的表达及临床意义

Expression and clinical significance of Six4 in colorectal carcinoma tissues

目的:探索Six4蛋白在结肠癌中的表达及其与临床特征的关联性.方法:通过医学癌症数据库分析Six4表达与结肠癌患者生存时间的相关性;利用组织芯片,通过免疫组化检测结肠癌组织及癌旁组织中Six4蛋白的表达情况,统计分析其表达差异,分析Six4蛋白表达与结肠癌患者的临床特征及预后的关系.结果:Six4高表达结肠癌患者生存期较低表达患者明显缩短(P=0.002).在结肠癌组织中Six4蛋白表达显著高于癌旁组织(P<0.001).Six4表达水平与结肠癌患者病理分期(P=0.020)、肿瘤大小(P=0.034)、MSH6表达水平(P=0.035)有相关性,而与性别、年龄、淋巴结转移、是否远处转移、TNM分期、Ki67、P53、MSH2蛋白表达水平无明显相关性.Six4蛋白的表达水平(P=0.029)可以作为结肠癌患者的独立预后因素.结论:Six4蛋白高表达与不良预后相关;Six4蛋白在结肠癌组织较癌旁组织高表达;Six4蛋白与病理分期、肿瘤大小、MSH6表达水平相关.

Objective:To explore the expression and significance of Six4 in colorectal carcinoma tissues.Methods:Analyze the relationship between Six4 expression and survival time of CRC patients.The immunohistochemical SP was used to detect the expression of Six4 protein in CRC tissues and adjacent tissues,statistically analyzed the different expression and the relationship between Six4 protein expression and clinical factors and prognosis of CRC.Results:The survival time of CRC patients with high Six4 expression was significantly shorter than that of patients with low Six4 expression(P=0.002).The expression of Six4 protein was significantly higher in CRC than in adjacent normal tissues.Six4 expression was significantly correlated with pathological stage(P=0.020),tumor size(P=0.034),the expression level of MSH6(P=0.035),but not with gender,age,lymph node metastasis,distant metastasis,TNM stage,expression levels of Ki67,P53 and MSH2.The expression level of Six4(P=0.029)was independent prognostic factors for CRC patients.Conclusion:High expression of Six4 protein was associated with poor prognosis.Six4 was highly expressed in CRC tissues compared to adjacent normal tissues;With Pathological stage,tumor size,MSH6 expression level related,the expression level of Six4 served as an independent prognostic factor for patients with CRC.Six4 protein plays an important role in the development of CRC.