富马酸钠对荧光假单胞菌群体感应现象及其腐败活性的抑制作用

Inhibitory Effect of Sodium Fumarate on Quorum Sensing and Spoilage Capacity of Pseudomonas fluorescens

以报告菌株紫色杆菌CV026为检测模型,探究富马酸钠对荧光假单胞菌群体感应现象及其腐败特性的影响。采用酶标法及光学显微镜观察富马酸钠对荧光假单胞菌生物被膜的抑制效果,利用气相色谱-质谱分析富马酸钠对荧光假单胞菌分泌信号分子的影响。结果表明:在亚抑菌浓度下,富马酸钠可以显著降低紫色杆菌CV026紫色菌素的产生量(P<0.05);当质量浓度为2.0 mg/mL时,富马酸钠对紫色菌素的抑制率达50.95%,且不影响CV026菌株的生长。富马酸钠能有效抑制荧光假单胞菌生物被膜形成、群集与泳动、胞外蛋白酶活性等相关腐败特性,且质量浓度与其抑制作用呈正相关。另外,由气相色谱-质谱检测结果可知,富马酸钠对荧光假单胞菌N-酰基高丝氨酸内酯信号分子的分泌有较好的抑制效果。因此,富马酸钠具有较好的群体感应抑制活性,可作为新型群体感应抑制剂用于水产品贮藏保鲜。

In this paper, Chromobacterium violaceum CV026 strain was used as a test model to investigate the effect of sodium fumarate on quorum sensing and spoilage capacity of Pseudomonas fluorescens. The inhibitory effect of sodium fumarate on biofilm formation was evaluated with a microplate reader and optical microscopy, and its effect on the secretion of signal molecules in Pseudomonas fluorescens was determined by gas chromatography-mass spectrometry(GC-MS). The results showed that sodium fumarate could significantly reduce the production of violacein in strain CV026 at sub-minimum inhibitory concentrations. Violacein production was inhibited 50.95% by sodium fumarate at 2.0 mg/mL while not affecting the growth of CV026. Sodium fumarate could effectively inhibit spoilage characteristics of Pseudomonas fluorescens as reflected by biofilm formation, swarming and swimming motility, and extracellular protease activity and this effect was positively correlated with its concentration. By GC-MS analysis, we also found that sodium fumarate significantly inhibited the secretion of the signal molecules N-acylhomoserine lactones(AHLs). Therefore,sodium fumarate has great inhibitory activity and can be developed as a quorum sensing inhibitor for refrigerated storage and preservation of aquatic products.