五味子蜂花粉不同萃取物对小鼠肝脏脂质过氧化及DNA氧化损伤的作用

Effects of Different Solvent Extracts from Schisandra chinensis Bee Pollen on Hepatic Lipid Peroxidation and DNA Oxidative Damage

五味子蜂花粉具有强抗氧化活性,为了探究其抗氧化活性成分分布,本研究采用体积分数75%乙醇溶液对五味子蜂花粉进行浸提,分别用石油醚、乙酸乙酯和正丁醇进行萃取,测定不同萃取物的活性成分和体外抗氧化活性,研究其对小鼠肝脏脂质过氧化的抑制作用和对·OH诱导的pBR322质粒DNA氧化损伤的保护作用。结果表明,五味子蜂花粉不同萃取物抗氧化活性及其对小鼠肝脏脂质过氧化的抑制作用和对DNA氧化损伤的保护作用均存在显著差异(P<0.05),其顺序为:正丁醇萃取物>乙酸乙酯萃取物>石油醚萃取物。正丁醇萃取物的总酚含量最高,为26.11g/100g,总黄酮含量为27.90g/100g;有很强的Fe2+络合能力、还原力和对1,1-二苯基-2-三硝基苯肼自由基的清除能力;0.2mg/mL正丁醇萃取物对小鼠肝脏脂质过氧化的抑制率可达85%以上。本研究为进一步分离纯化五味子蜂花粉高抗氧化活性酚类物质提供依据,同时为抗氧化功能食品的开发提供参考。

Schisandra chinensis bee pollen has strong antioxidant activity. In order to explore the distribution of antioxidant components in it, the 75% ethanol extract of S. chinensis bee pollen was fractionated by successive extraction with petroleum ether, ethyl acetate and n-butanol. The active ingredients and antioxidant activity in vitro of the fractions were evaluated as well as the inhibitory effect on lipid peroxidation of liver homogenate and the protective effect on DNA oxidative damage. The results showed that the fractions differed significantly with respect to their antioxidant activity, inhibitory effect on lipid peroxidation and protective effect on oxidative DNA damage(P < 0.05), in the decreasing order: n-butanol extract > ethyl acetate extract > petroleum ether extract. The n-butanol extract had the highest total phenolic content(26.11 g/100 g), and its total flavonoid content was 27.90 g/100 g. This extract had strong Fe2+ chelating power, reducing power, and 1,1-diphenyl-2-picrylhydrazyl scavenging capacity. Moreover, 85% of lipid peroxidation was inhibited by 0.2 mg/mL of the n-butanol extract. This study provides a basis for the separation and purification of powerfully antioxidant phenolics from S. chinensis bee pollen and for the development of antioxidant foods.