普洱茶对膳食诱导肥胖大鼠降低体质量及调节细胞因子的作用

Pu-erh Tea Inhibits Body Mass Gain and Modulates Cytokine Profiles of Nutritionally Obese Sprague-Dawley Rats

为探究普洱茶的减肥机理和保健功效,本研究以正常体质量的雄性SD大鼠作为正常对照组,将膳食诱导的肥胖SD大鼠随机分为5组(每组8只):肥胖对照组,西布曲明阳性对照组和低、中、高剂量普洱茶处理组,每天灌胃给药,每周测体质量,连续给药42 d后禁食24 h,眼底取血,测定血清中的葡萄糖、胰岛素、瘦素、脂联素、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、白细胞介素(interleukin,IL)-1α、IL-1β、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high-densitylipoproteincholesterol,HDL-C)的水平。结果表明:低、中、高剂量普洱茶处理组与肥胖对照组相比,大鼠体质量分别显著下降了24.80%、26.59%和27.62%(P<0.05);LDL-C浓度显著下降了7.11%、9.11%和25.74%(P<0.05),葡萄糖浓度极显著下降了57.3%、67.1%、72.7%(P<0.01),胰岛素含量下降了8.6%、17.2%、41.7%,瘦素质量浓度极显著下降了68.6%、66.8%和64.6%(P<0.01),VEGF质量浓度极显著下降了45.3%、44.6%、40.5%(P<0.01),TNF-α质量浓度极显著下降了25.0%、27.8%、33.3%(P<0.01);HDL-C浓度显著升高了20.4%、22.2%、25.0%(P<0.05);脂联素质量浓度显著增加了5.9%、7.6%和9.7%(P<0.05),IL-1α质量浓度升高,IL-1β质量浓度降低,调节了IL-1α/IL-1β的比例,因此,普洱茶对高脂饮食诱导的肥胖大鼠具有减轻体质量和调节细胞因子的作用。

In order to explore the antiobesity mechanism and health benefits of Pu-erh tea, SD rats with normal body mass were used as the normal control group, and nutritionally induced obese SD rats were randomly divided into five groups of8 rats each: obese model group, sibutramine positive control group, and low-, middle-, and high-dose Pu-erh tea groups.The rats were administered with Pu-erh tea by gavage for 42 successive days, and their body masses were measured once a week. Glucose(Glu), insulin, leptin, adiponectin, tumor necrosis factor-alpha(TNF-α), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C), interleukin-1 alpha/beta(IL-1α/β) and vascular endothelial growth factor(VEGF) levels were determined in the serum of rats in each group after 24 h fasting. Compared with the obese control,the body mass in the low-, middle-, and high-dose Pu-erh tea groups decreased by 24.8%, 26.59% and 27.62%(P < 0.05),respectively, serum Glu by 57.3%, 67.1% and 72.7%(P < 0.01), LDL-C by 7.11%, 9.11% and 25.74%(P < 0.05), insulin by 8.6%, 17.2% and 41.7%, leptin by 68.6%, 66.8%, 64.6%(P < 0.01), VEGF by 45.3%, 44.6%, 40.5%(P < 0.01), and TNF-α by 25.0%, 27.8%, 33.3%(P < 0.01);whereas HDL-C significantly increased by 20.4%, 22.2% and 25.0%(P < 0.05),and adiponectin by 5.9%, 7.6% and 9.7%(P < 0.05), respectively. Moreover, Pu-erh tea increased IL-1α and reduced IL-1β, thereby modulating the ratio of IL-1α to IL-1β. Therefore, Pu-erh tea obviously reduces body mass gain and modulates cytokine profiles in high-fat diet-induced obese rats.