CaCl2增强橄榄假丝酵母对苹果果实采后青霉病的生防效力

CaCl2 Enhances the Biocontrol Efficacy of Candida oleophila against Inoculated Penicillium expansum on Postharvest Apples

采用同孔接种的方法研究橄榄假丝酵母(Candida oleophila)结合CaCl2处理对采后苹果青霉病的控制效果及相关机理。结果表明:采用1×107 CFU/mL橄榄假丝酵母结合10 g/L CaCl2处理苹果果实,对其采后青霉病有显著的控制效果,且效果明显优于橄榄假丝酵母单独处理;在25℃条件下培养,橄榄假丝酵母能在果实伤口处迅速定植生长,并持续保持较高水平,10 g/L CaCl2对橄榄假丝酵母生长状态有明显促进作用;同时,橄榄假丝酵母复合CaCl2处理能显著诱导苹果果实几丁质酶和β-1,3-葡聚糖酶活力的提高,促进相关代谢产物木质素、总酚、类黄酮的积累。

The effect and underlying mechanism of Candida oleophila combined with CaCl2 on the biocontrol efficiency of Penicillium expansum inoculated onto postharvest apples were studied in this paper. The results showed that the effect of combined treatment with 1 × 107 CFU/mL C. oleophila and 10 g/L CaCl2 on the control of Penicillium expansum was significantly better than that of C. oleophila. When cultured at 25 ℃, C. oleophila could quickly colonize and grow on wounded apples and maintain a high population. The treatment with 10 g/L CaCl2 could significantly enhance the growth of C. oleophila. Moreover, under the combined treatment, the activities of chitinase and β-1,3-glucanase were significantly induced;meanwhile, the accumulation of lignin, total phenols and flavonoids as metabolites was promoted.