萝卜蚜有翅型和无翅型转录组差异分析

Transciriptomic analysis of winged and wingless Lipaphis erysimi(Kaltenbach)

采用Illumina测序平台获得了萝卜蚜转录组数据,有翅蚜和无翅蚜混合样本共得到107992806条CleaningReads,组装得到69588条Unigene序列,平均长度为716bp。有翅蚜测序结果共得到44620184条CleaningReads;无翅萝卜蚜测序结果共得到47113716条CleaningReads。2组样品差异表达基因的分析表明共有显著性差异表达基因104个,其中表达上调基因有74个,表达下调基因有30个。对差异表达基因进行GO注释及KEGG富集分析显示,Hippo信号通路、甲状腺激素信号通路和催产素信号通路与翅型分化及与之紧密联系的生殖率差异相关性较强。

In this study,the transcriptome of winged and wingless Lipaphis erysimi was obtained with Illumina sequencing platform. A total of 107 992 806 cleaning reads were obtained from the mixed samples,and were assembled into 69 588 unigenes with the average length of 716 bp. A total of 44 620 184 and 47 113 716 Cleaning Reads were obtained from the winged and wingless aphid samples,respectively. Analysis of gene expression between the winged and wingless samples showed 104 significantly differentially expressed genes,of which 74 genes were up-regulated and 30 genes were down-regulated. Subsequently,GO annotation and KEGG pathway enrichment analysis showed that among those differentially expressed genes,Hippo signaling pathway,Thyroid hormone signaling pathway and Oxytocin signaling pathway were strongly related to the wing dimorphism and its related reproduction.