补肾调周法联合清利化瘀中药灌肠治疗盆腔炎性疾病后遗症—慢性盆腔痛的疗效及对辅助性T细胞1/辅助性T细胞2平衡的调节作用

Therapeutic effect of Bushen Tiaozhou method combined with Qingli Huayu traditional Chinese medicine enema on sequelae of pelvic inflammatory disease-chronic pelvic pain and its regulating effect on balance of helper T cell 1/helper T cell 2

目的观察补肾调周法联合清利化瘀中药灌肠治疗盆腔炎性疾病后遗症-慢性盆腔痛湿热瘀结证的疗效及对辅助性T细胞1(Th1)/Th2平衡的调节作用。方法将110例盆腔炎性疾病后遗症-慢性盆腔痛湿热瘀结证患者按随机数字表法分为2组。对照组55例(脱落1例)予盐酸左氧氟沙星胶囊联合替硝唑胶囊口服治疗,治疗组55例在对照组治疗基础上加补肾调周法中药口服联合清利化瘀中药保留灌肠治疗。2组均治疗3个月经周期后统计临床疗效。比较2组治疗前后中医证候评分、外周血Th1和Th2细胞占CD4^+T细胞比例及血清白细胞介素2(IL-2)、IL-6、IL-10、肿瘤坏死因子α(TNF-α)水平变化。结果治疗后,2组各中医证候评分均较本组治疗前降低(P<0.05),且治疗组低于对照组(P<0.05)。治疗后,2组Th1细胞比例及Th1/Th2均较本组治疗前增加(P<0.05),Th2细胞比例减少(P<0.05),且2组组间比较差异均有统计学意义(P<0.05)。治疗后,2组血清IL-2、IL-10水平较本组治疗前升高(P<0.05),IL-6、TNF-α水平降低(P<0.05),且2组组间比较差异均有统计学意义(P<0.05)。治疗组总有效率(94.54%)高于对照组(79.63%,P<0.05)。结论补肾调周法联合清利化瘀中药灌肠治疗盆腔炎性疾病后遗症-慢性盆腔痛湿热瘀结证,可促进症状改善,提高疗效,其疗效途径可能与调节Thl/Th2细胞平衡有关。

Objective To observe the therapeutic effect of Bushen Tiaozhou method combined with Qingli Huayu traditional Chinese medicine enema on sequelae of pelvic inflammatory disease-chronic pelvic pain and its regulating effect on balance of helper T cell 1/helper T cell 2. Methods 110 patients with sequelae of pelvic inflammatory disease-chronic pelvic pain with dampness and heat stasis syndrome were divided into two groups according to the random number table method. The control group(55 cases, 1 case of abscission) were treated by levofloxacin hydrochloride capsules combined with tinidazole capsules for oral treatment. The treatment group(55 cases) were treated by Bushen Tiaozhou method of traditional Chinese medicine oral administration combined with Qingli Huayu traditional Chinese medicine retention enema on the basis of the control group. The clinical efficacy of the two groups were statistical after 3 menstrual cycles. The changes of TCM syndrome scores, Th1 and Th2 cells in peripheral blood accounted for the proportion of CD4^+T cells, and serum interleukin 2(IL-2), IL-6, IL-10, tumor necrosis factor α(TNF-α) before and after treatment between the two groups were compared. Results After treatment, the scores of TCM syndromes in two groups were lower than those in the group before treatment(P<0.05), and the treatment group was lower than the control group(P<0.05). After treatment, the ratio of Th1 cells and Th1/Th2 cells in the two groups were increased(P<0.05), and the ratio of Th2 cells was decreased(P<0.05), and there were statistically significant differences between the two groups(P<0.05). After treatment, the levels of serum IL-2 and IL-10 in the two groups were higher than those in the group before treatment(P<0.05), and the levels of IL-6 and TNF-α were decreased(P<0.05), there were statistically significant differences between the two groups(P<0.05). The total effective rate of the treatment group(94.54%) was higher than that of the control group(79.63%, P<0.05). Conclusion Treatment of sequelae of pelvic inflammatory disease by Bushen Tiaozhou method combined with Qingli Huayu traditional Chinese medicine enema-chronic pelvic pain with dampness and heat stasis syndrome, It can promote the improvement of symptoms and improve the curative effect, and its therapeutic effect may be related to the regulation of cell balance of Th1/Th2 cells.