Design and synthesis of nitrogen-fused pyridazinone fluorescent probes and their application in biological imaging

氮杂芳香环并哒嗪酮类荧光探针的设计、合成和生物成像的应用（英文）

A series of small-molecular fluorescent probes based on nitrogen-fused pyridazinone scaffold were developed in this report.The design strategy involved two steps:1)enhancing the electron-withdrawing ability of the acceptor by incorporating an N-heterocyclic aromatic ring(pyridine or pyrazine)at the C4 and C5 positions of the pyridazinone skeleton and 2)anchoring a triphenylphosphine or morpholine tail as the subcellular targeting group.These fluorescent probes displayed excellent properties in live cell and brain tissue imaging.

本文合成了一系列氮杂芳香环并哒嗪酮的小分子荧光探针。设计策略主要包括以下两点:1)通过在哒嗪酮4, 5-C上并入含氮芳香杂环(吡啶或吡嗪),增强电子受体的吸电子能力; 2)末端引入细胞器靶向基团三苯基膦或吗啡啉。这些探针在活细胞成像和脑组织成像上均表现出优良的性质。