摘要
本研究建立了一种同时鉴定甘西鼠尾草(Salviaprzewalskii)中的15种化学成分的高效液相色谱-二极管阵列(HPLC-DAD)检测方法。该方法具有良好的线性、精密性、稳定性和回收率。采用HPLC-DAD建立了甘西鼠尾草的指纹图谱,以迷迭香酸为参照,确定了17个共有峰。基于相似系统理论,10份样品的相似性指数均高于0.948,表明共有峰之间具有良好的相关性。利用甘西鼠尾草的指纹图谱从丹参(Salviamiltiorriza)中鉴别出了甘西鼠尾草,并可确定甘西鼠尾草的地理起源。结果表明,该方法可作为甘西鼠尾草的质量控制方法。
In the present study,we developed a novel high-performance liquid chromatography-diode array detection(HPLC-DAD)method for the simultaneous determination and fingerprinting of 15 components in Salvia przewalskii.The method had good linearity,precision,stability and recovery.Chromatographic fingerprints were determined by HPLC-DAD using rosmarinic acid peaks as references,and 17 common peaks were selected.The similarity indexes calculated based on similarity system theory of the 10 samples were higher than 0.948,indicating good correlation among the common peaks.The chromatograms distinguished S.przewalskii from Salvia miltiorrhiza and identified the origins of S.przewalskii.These results suggested that the proposed method was suitable for S.przewalskii quality control.
作者
姜媛媛
王涛
张慧
陈欢
王龙
张利
周永红
Yuanyuan Jiang;Tao Wang;Hui Zhang;Huan Chen;Long Wang;Li Zhang;Yonghong Zhou(College of Sciences,Sichuan Agricultural University,Ya' an 625014,Sichuan,China;Institue of Chinese Medicinal Materials,Mianyang Academy of Agriculural Sciences,Mianyang 621023,Sichuan,China;Sichuan University,West China School of Pharmacy,Chengdu 610041,Sichuan,China;Triticeae Research Institute,Sichuan Agriculural Universiy,Wenjiang 611130,Sichuan,China)
基金
The Program of Chinese Herbal Medicines Breeding Research of Sichuan(Grant No.2016NYZ0036-3-3)
the Program of Sichuan Science and Technology Conditional Platform Construction(Grant No.2018TJPT0013)
关键词
甘西鼠尾草
酚酸
丹参酮
定量分析
指纹图谱
Salvia przewalskii
Phenolic acid
Tanshinone
Quantitative analysis
Fingerprints
