肥胖中医体质与单核苷酸多态性的关系研究

Study on the relationship between obesity constitution of traditional Chinese medicine and single nucleotide polymorphisms

目的探究肥胖人群中医体质的分布情况,进一步研究肥胖中医体质与肥胖FTO基因单核苷酸多态性的关系。方法收集健康人群/肥胖人群样本量分别为48、52例,测量体质量指数(BMI)、采外周血标本,采用改良多重高温连接酶检测反应技术(iMLDR)检测FTO基因上的3个SNP位点(rs9939609、rs1421085、rs8057044)。使用对其中的肥胖人群进行中医学体质分类。对健康人群和肥胖人群的FTO基因SNP位点进行分析,将健康人群/肥胖人群SNP突变结果、肥胖人群不同中医体质SNP突变结果进行统计学分析。结果52例肥胖组人群中,按照中医体质由多到少依次为:肝郁气滞型、脾虚湿阻型、胃热湿阻型、脾肾两虚型、阴虚内热型,全身脂肪含量(FM)和体脂百分比上,5种体质差异无统计学意义(P>0.05)。在rs9939609中基因型及等位基因的概率经比较,差异无统计学意义(P>0.05);rs1421085中C基因及CC+CT基因型分布频率高于对照组,两组间差异有统计学意义(P<0.05);在rs8057044中A基因及AA+AG基因型分布频率高于对照组,两组间差异有统计学意义(P<0.05)。rs1421085的CT与CC基因型在脾虚湿阻型中大量分布,概率分别为61.53%、23.08%。rs8057044的AG与AA基因在脾肾两虚型中大量分布,概率分别为:50%、50%。rs8057044的AG与AA基因阴虚内热型中大量分布,概率分别为75%、25%。结论FTO基因的rs9939609的A基因在肥胖组和对照组中未见明显差异,且不推荐作为中医体质分型的指标,rs1421085与脾虚湿阻型高度相关,rs8057044与脾肾两虚型和阴虚内热型高度相关。

Objective To explore the distribution of traditional chinese medicine (TCM) constitution symptoms in obese people,and further study the relationship between TCM constitution and FTO single nucleotide polymorphism of obesity gene. Methods 48 healthy people and 52 obese people were enrolled.Body mass index (BMI) was measured and peripheral blood samples were collected.Three SNP loci (rs9939609,rs1421085,rs8057044) in the FTO gene were detected by improved multiple hyperthermic ligase assay (iMLDR).The TCM constitution classification of obese people was carried out.The SNP loci of FTO gene in healthy and obese people were analyzed.The results of SNP mutation in healthy and obese people and in obese people with different TCM constitutions were statistically analyzed. Results Among 52 obese people,according to the number of constitutional people in TCM,the order from the large to small was liver-qi stagnation type,spleen-deficiency-dampness type,stomach-heat-dampness type,spleen-kidney deficiency type and yin-deficiency-internal-heat type.There was no significant difference in body fat percentage FM and body fat percentage between the five TCM constitutions (P >0.05).The probability of genotype and allele in rs9939609 had no statistical significance (P >0.05),and the frequency of C gene and CC+CT genotype in rs1421085 was higher than that in the control group,and there was statistical significance between the two groups (P<0.05).The frequency of A gene and AA+AG genotype in rs8057044 was higher than that in the control group,and there was statistical difference between the two groups (P<0.05).The CT and CC genotypes of rs1421085 were largely distributed in spleen-deficiency-dampness type,with a probability of 61.53% and 23.08 % respectively.The AG and AA genes of rs8057044 were largely distributed in spleen-kidney deficiency type,with a probability of 50% and 50% respectively.The AG and AA genes of rs8057044 were largely distributed in yin-deficiency-internal-heat type,with a probability of 75% and 25% respectively. Conclusion The rs9939609 of A gene of FTO gene was not significantly different between the obese group and the control group,and was not recommended as an indicator of TCM constitution type.rs1421085 was highly correlated with spleen deficiency dampness type.rs8057044 was highly correlated with spleen-kidney deficiency type and yin-deficiency-internal-heat type.