干扰素α刺激角质形成细胞表达HLA-I/II和CD40分子的研究

Interferon alpha stimulates expression of keratinocytes HLA-I/II and CD40 molecules

目的:探究干扰素(interferon,IFN)α刺激角质形成细胞后,角质形成细胞表面共刺激分子CD40、CD80、CD86和提呈相关分子HLA-I、HLA-DR的变化以及IFN-α对角质形成细胞生存曲线的影响。方法:以人永生化口腔黏膜角质形成细胞(human immortlized oral mucosal keratinocytes,HOK)和Ha Ca T细胞为细胞模型,以IFN-α(实验组)、IFN-γ(阳性对照组)、PBS(阴性对照组)分别刺激细胞,以流式细胞术检测细胞表面CD40、CD80、CD86、HLA-I、HLA-DR的变化。结果:与阴性对照组相比,IFN-α实验组的角质形成细胞在刺激后24 h,HLA-I类分子、HLA-DR和CD40的阳性细胞比例及平均荧光强度明显提高。结论:IFN-α可刺激角质形成细胞表达HLA-I类分子、HLA-DR和CD40分子。

Objective: To investigate the effects of IFN-α on the expression of co-stimulatory molecules CD40,CD80,CD86 and related molecules HLA-I and HLA-DR after IFN-α stimulation of keratinocytes and the survival curve of keratinocytes in IFN-α.Methods: Human immortalized oral mucosal keratinocytes and Ha Ca T cells were used as cell models,and IFN-α( experimental group),IFN-γ( positive control group),and PBS( negative control group) were used to stimulate cells to flow cells.The changes of CD40,CD80,CD86,HLA-I and HLA-DR on the cell surface were detected.Results: Compared with the negative control group,the proportion of HLA-I molecules,HLA-DR and CD40 positive cells and the average fluorescence intensity of keratinocytes in the IFN-α experimental group were significantly increased 24 hours after stimulation.Conclusion: IFN-α can stimulate keratinocytes to express HLA-I molecules,HLA-DR and CD40 molecules.