RTCB在小鼠不同组织中的表达

Expression of RTCB in different tissues of mice

目的检测RTCB在小鼠各种组织中的表达和分布。方法提取小鼠胃、前列腺、子宫、卵巢等组织的总蛋白,利用免疫印迹技术,检测RTCB在各组织的相对表达量;通过免疫组织化学技术,检测RTCB在各组织的表达定位情况。结果免疫印迹结果显示,小鼠17种组织中RTCB均有表达,输卵管、骨骼肌、肾脏的相对表达量较高,而在膀胱和小肠的相对表达量较低。免疫组织化学结果显示,RTCB在不同组织或同一组织的不同细胞中免疫染色存在差异,尤其在睾丸、附睾、卵巢、子宫、输卵管、小肠等组织某些特定细胞中免疫染色较强。结论 RTCB可能参与小鼠生殖系统功能的维持。

Objective To investigate the expression and distribution of RTCB in various tissues of mice. Methods We detected the expression of RTCB by Western blotting and the location of the expression of RTCB in each tissue by immunohistochemistry. Results RTCB protein was all expressed in 17 tissues of mice. The relative expressions of oviduct, skeletal muscle and kidney were high, while the relative expressions of bladder and small intestine were low. In addition, the immunostaining of RTCB was different in different tissues or in different cells of the same tissue. The immunostaining was stronger in some specific cells of testis, epididymis, ovary, uterus, fallopian tube and small intestine. Conclusion RTCB may participate in the maintenance of reproductive function in mice.