基于寒热证模型大鼠研究五倍子发酵百药煎的药性变化和归属

Transformation and attribution of drug properties in Galla Chinesis fermented Baiyaojian based on cold and heat syndrome rats

研究五倍子与百药煎对寒、热证模型大鼠的影响,揭示五倍子发酵为百药煎的药性变化和归属。建立左甲状腺素钠片灌胃给药诱导大鼠热证模型和冰水刺激诱导大鼠寒证模型,分别灌胃给予五倍子和百药煎不同剂量水煎液15 d,观察记录体征指标的变化,测定大鼠肝脏中丙酮酸(PA)含量和ATP酶活力,血清中大鼠多巴胺(DA)、甲状腺激素(T4)、磷酸腺苷(c AMP)、5-羟色胺(5-HT)、去甲肾上腺素(NE)、17-羟皮质类固醇(17-OHCS)、促甲状腺激素释放激素(TRH)和促甲状腺激素(TSH)含量。结果热证模型组大鼠肛温、饮食饮水量及体质量升高,模型组c AMP,NE,17-OHCS,TRH,PA含量显著升高(P<0. 05),TSH,Na-K ATPase,Ca-Mg ATPase含量较空白组极显著升高(P<0. 01),5-HT含量较空白组显著降低(P<0. 05);五倍子高剂量(WG)及百药煎高剂量组(BG) T4,DA,NE,TSH,TRH,c AMP,17-OHCS含量极显著降低(P<0. 01),PA,Ca-Mg ATPase较模型组含量显著降低(P<0. 05),且五倍子高剂量组(WG)含量低于百药煎高剂量组,但无显著性差异;五倍子高剂量组及百药煎高剂量组大鼠血清5-HT含量较模型组均升高,且五倍子高剂量组含量高于百药煎高剂量组,但无显著性差异;热证模型组大鼠各脏器指数与空白组比较未出现明显改变,给药组大鼠脏器指数与模型组比较,未见显著性差异。寒证模型组、五倍子组和百药煎组大鼠均出现肛温、饮食饮水量及体质量降低,DA,T4,c AMP,NE,17-OHCS,TRH,TSH,PA,Na-K ATPase,CaMg ATPase含量较空白组均显著降低(P<0. 05),5-HT含量较空白组均显著升高(P<0. 05),心、肺、肾脏指数较空白组显著升高(P<0. 05)。五倍子和百药煎均能明显改善左甲状腺素钠所致的热症模型大鼠症状,且五倍子的效果优于百药煎,但均不能改善冰水刺激引起的寒证模型大鼠症状,提示五倍子与百药煎药性同为寒凉,但百药煎寒性弱于五倍子,发酵炮制的百药煎寒性缓和,结合临床应用药性归属“微寒”比“平”更为准确。

To reveal the transformation and attribution of drug properties in Galla Chinesis fermented Baiyaojian by studying the effect of Galla Chinesis and Baiyaojian on cold and heat syndrome rats. Euthyrox was used to induce the hyperthyrosis model,ice water stimulation was used to induce the cold syndrome model,and different concentrations of Galla Chinesis and Baiyaojian water decoction were administrated by gavage for 15 d continuously. Symptom indexes were evaluated,content of pyruvic acid( PA),ATPase activity in liver and contents of DA,T4,cAMP,5-HT,NE,17-OHCS,TRH and TSH in serum were assayed by enzyme linked immunosorbent assay and spectrophotometry. The rectal temperature,water consumption and body weight of heat syndrome rats in model group were increased,cAMP,NE,17-OHCS,TRH and PA were increased,TSH,Na-K ATPase and Ca-Mg ATPase were increased significantly( P<0. 01),while 5-HT was decreased,compared with those of the blank group( P< 0. 05),the contents of T4,DA,NE,TSH,TRH,cAMP and 17-OHCS were decreased significantly( P<0. 01),PA and Ca-Mg ATPase in WG and BG groups were decreased compared with those of the model group( P<0. 05),and the Galla Chinesis content of WG group was lower than that of BG group,while the contents of 5-HT in WG and BG groups were increased,and the Galla Chinesis content of WG group was higher than that of BG group,with no significant difference of viscera index between heat syndrome rats in blank group,model group and drug groups. The rectal temperature,water consumption and body weight of cold syndrome rats in model group were decreased,DA,T4,cAMP,NE,17-OHCS,TRH,TSH,PA,Na-K ATPase and Ca-Mg ATPase of rats in model group were decreased,whereas 5-HT was increased compared with those of the blank group( P<0. 05),the indexes of heart,lung and kidney were significantly higher than those in the blank group( P<0. 05). Both Galla Chinesis and Baiyaojian can significantly alleviate the symptoms of heat syndrome rats caused by levothyroxine sodium. Galla Chinesis has a better effect than Baiyaojian,but cannot alleviate the symptoms of cold syndrome caused by ice water stimulation,suggestting that the decoction of Galla Chinesis and Baiyaojian are both cold,but Galla Chinesis is colder than Baiyaojian. Cold property in Galla Chinesis fermented Baiyaojian can be relieved. In clinical application,the property of " slight cold" is more accurate than " neutral property" for Baiyaojian.