髓细胞性白血病患儿PTEN蛋白表达及其与免疫表型的关系

Expression of PTEN Protein and Its Relationship with Immunophenotype in Children with Myeloid Leukemia

目的:探讨髓细胞性白血病(AML)患儿PTEN蛋白表达及其与免疫表型的关系。方法:选择AML患儿143例,根据免疫表型的不同分为两组:免疫分型为LY+AML型59例,LY-AML84例。所有患儿都给予免疫表型分析,检测PTEN蛋白表达情况并进行相关性分析。结果:LY+AML患儿CD34^+阳性、CD117^+阳性比例显著高于LY-AML患儿(P <0.05),染色体核型异常比例显著低于LY-AML患儿(P <0.05)。LY+AML患儿的PTEN蛋白表达量为(65.33±2.34)%,阳性表达率为94.9%;而LY-AML分别为(20.11±4.11)%和13.1%,与LY+AML患儿对比差异都有统计学意义(P <0.05)。在AML患儿中,Spearman相关分析显示PTEN蛋白表达水平与免疫分型呈现显著相关性(r=0.653,P=0.000)。多因素logistic回归方法显示PTEN蛋白表达、CD34^+阳性、CD117^+阳性、染色体核型异常为影响AML患儿免疫表型的主要独立危险因素(OR=1.098、1.045、1.092、0.294,P <0.05)。结论:AML患儿骨髓单个核细胞的PTEN蛋白表达上调使得LY+AML型发生风险显著增加,可作为AML患儿病情判断与预后预测的参考指标之一。

Objective: To investigate the expression of PTEN protein and its relationship with immunophenotype in children with acute myeloid leukemia(AML). Methods: 143 children with AML were selected. According to the different of immunophenotype, they were divided into two groups: 59 cases of LY+AML type and 84 cases of LY-AML. All the children were given immunophenotypic analysis, and the expression of PTEN protein were detected and given the correlation analysis. Results: The proportion of CD34^+positive and CD117^+positive in children with LY+AML were significantly higher than that in children with LY-AML(P<0.05), and the abnormal proportion of karyotype were significantly lower than that in children with LY-AML(P <0.05). The expression of PTEN protein in children with LY+AML were(65.33±2.34)%, and the positive expression rates were 94.9%. The LY-AML were(20.11±4.11)% and13.1%, respectively, compared he difference were statistically significant in children with LY+AML(P <0.05). In children with AML,Spearman correlation analysis showed a significant correlation between PTEN protein expression and immunophenotyping(r =0.653,P=0.000). Multivariate logistic regression showed that PTEN protein expression, CD34^+positive, CD117^+positive, and karyotypic abnormalities were the main independent risk factors affected the immunophenotype of children with AML(OR =1.098, 1.045, 1.092,0.294, P <0.05). Conclusion: The upregulation of PTEN protein expression in the bone marrow mononuclear cells can significantly increase the risk of LY+AML. It can be used as the reference index of the prognosis of children with AML.