镍钛合金纳米管阵列调控内皮细胞miRNA的表达研究

Regulation of miRNA expression in endothelial cells induced by nitinol nanotubular array

目的探究镍钛合金纳米管阵列对内皮细胞微小RNA(miRNA,miR)表达的影响。方法在镍钛合金光滑对照与纳米管阵列表面培养人原代冠状动脉内皮细胞(HCAEC),提取不同样品表面细胞RNA进行miRNA芯片表达谱分析,筛选出差异表达miRNA,进一步对差异表达miRNA进行靶基因预测。同时,对靶基因进行基因本体论(GO)和信号通路分析。结果共筛选出25个差异表达miRNA,其中包括21个表达上调和4个表达下调的miRNA(FC≥2.0,P≤0.05)。差异表达miRNA靶基因富集GO条目60条,信号通路20条。结论镍钛合金纳米管阵列可能通过调控差异表达miRNA参与调控肌动蛋白细胞骨架、Notch、磷脂酰肌醇等重要信号通路,最终影响内皮细胞行为与功能。

Objective To investigate the effect of nanotubular arrays on the expression of microRNAs(miRNAs)in endothelial cells(ECs)in order to explore the molecular mechanisms by which ECs respond to nanotubular arrays. Methods Human coronary artery endothelial cells(HCAECs)were cultured on the surface of smooth nitinol and nitinol-based nano-tubular arrays,respectively. miRNA microarray expression profiles were analyzed while differentially expressed miRNAs were screened. Target gene prediction of differentially expressed miRNAs was performed. Then,gene ontology(GO)and KEGG pathways were conducted. Results A total of 25 differentially expressed miRNAs were screened,21 of which were up-regulated and 4 were down-regulated(FC≥2.0 and P≤0.05). Target genes of differentially expressed miRNAs were enriched in 60 GO items and 20 KEGG pathways. Conclusion Nanotubular arrays may participate in the regulation of differentially expressed miRNAs involved in actin cytoskeleton,notch signaling pathways,and the phosphatidylinositol signaling system,which ultimately affects ECs behaviors and functions.